Auto-Mag® Tissue Total RNA Isolation Kit

Auto-Mag® Tissue Total RNA Isolation Kit is specially designed for purifying total RNA from a wide variety of tissues, cultured cells, and whole blood. The kit uses the special lysis condition with Auto-Mag® magnetic particles technology to isolate high-quality total RNA from 5-30mg of tissue, or 1 x 10⁶ of cultured cells. Purified RNA is suitable for all major downstream applications such as RNA-Seq, RT-PCR, NGS, and hybridization applications.

Highlights & Applications

Highlights

• Fast and easy processing using a magnetic bead system
• High yield and quality total RNA isolation and purification
• No toxic organic solvents
• Eluted RNA is well suitable for wide range of downstream applications
• Adaptable to various automated liquid handling workstations

Application

Total RNA isolation for:

• RT-qPCR
• RNA sequencing
• cDNA synthesis
• Gene expression analysis

Product Documents

Auto-Mag® Total RNA Isolation Kit – Protocol

Auto-Mag® Total RNA Isolation Kit – MSDS

Additional Information

Working in RNase Free Conditions

RNA purity and integrity is essential for downstream applications. RNA can be degraded by RNase A, which is a highly stable contaminant found in any laboratory environment. Some general precautions should be followed to avoid the introduction of contaminating nucleases especially during wash and elution steps. The most common sources of RNase contamination are hands, dust particles, and contaminated laboratory instruments, solutions, and glassware. The following procedures should be followed to limit RNase contamination when working with RNA:

• Always wear gloves while working and change gloves frequently
• Refrain from using reagents, consumables and equipment that are in common use for other general lab processes
• Use dedicated RNase free equipment such as pipettes, pipette tips, gels boxes, etc.
• Work in a separate room, fume hood or lab space if available
• Use plastic, disposable consumables that are certified RNase free
• Purchase reagents, such as commonly used buffers and water, that are certified RNase free. Prepare small individual aliquots of such buffers to avoid repeated transfer out of stock buffers. This lowers the risk of contamination of the stock solution
• Wipe down work surfaces with commercial RNase inhibiting surfactant solutions or 70% ethanol before starting work
• Keep the RNA on ice after extraction and while working with it.
• Store the extracted RNA at -20°C. For long term stability, keep the RNA at -80°C.

Certification of Analysis (COA)

Lot No. R02344208           Lot No. R02344747