Login

Register

Login

Register

Products

Auto-Mag® Dry Blood Spot DNA Isolation Kit
December 31, 2019
Auto-Mag® D-1 Particles
January 30, 2020

Auto-Mag® Plant Total RNA Isolation Kit

$120.00$380.00

Magnetic bead-based kit designed to extract high-quality total RNA from plant samples

Free Sample Kit upon request!

Catalog Item Description Price Quantity
R019-01 Auto Mag®Plant Total RNA Isolation Kit (50 Preps) $120.00
R019-02 Auto Mag®Plant Total RNA Isolation Kit (200 Preps) $380.00
View Cart
spinner-2x

Clear
SKU: AMD-R019 Categories: ,

Auto-Mag® Plant Total RNA Isolation Kit is specially designed for rapid and efficient purification of high-quality total RNA from a wide variety of plant species and tissue types. The kit utilizes Magnetic bead-based technology, and the standard procedure takes less than 30 minutes following cell lysis. The isolation can be performed in both 96-well plates (manually and automated) and in 1.5 ml tubes (manually only) with related protocols. Plant samples are disrupted in a homogenizer/bead-based milling equipment first. PRL Buffer is added to lyse the samples. The supernatant is then transferred to a new processing plate where Auto-Mag® C-5 beads are added to bind to the RNA. Following DNA are digested and a few wash steps, the highest integrity purified RNA is eluted and can be used in a wide range of downstream applications, such as Real-time PCR, Southern blotting, SNP analysis, NGS, hybridization applications, and other RNA-based analysis. The Auto-Mag® Plant Total RNA Isolation Kit can be adapted to high throughput liquid handling workstations.

 

Don't miss out!
Subscribe To Newsletter
Receive top education news, lesson ideas, teaching tips and more!
Invalid email address
Give it a try. You can unsubscribe at any time.

Highlights & Applications

Highlights

  • Fast and easy processing using a magnetic bead system
  • Robust lysis system – chemical lysis combined with a mechanical homogenization
  • Consistent, high yields of total RNA
  • Isolate high-quality total RNA from a variety of plant species.
  • Eluted RNA is well suitable for wide range of downstream applications

Application

 Plant Total RNA isolation for:

  • PCR, Real-time PCR
  • Cloning, genotyping
  • Sequencing
  • Plant breeding
Don't miss out!
Subscribe To Newsletter
Receive top education news, lesson ideas, teaching tips and more!
Invalid email address
Give it a try. You can unsubscribe at any time.

Product Documents

Auto-Mag® Plant Total RNA Isolation Kit – Protocol

Auto-Mag®Plant Total RNA Isolation Kit – MSDS

Don't miss out!
Subscribe To Newsletter
Receive top education news, lesson ideas, teaching tips and more!
Invalid email address
Give it a try. You can unsubscribe at any time.

Additional Information

Working in RNase Free Conditions

RNA purity and integrity is essential for downstream applications. RNA can be degraded by RNase A, which is a highly stable contaminant found in any laboratory environment. Some general precautions should be followed to avoid the introduction of contaminating nucleases especially during wash and elution steps. The most common sources of RNase contamination are hands, dust particles, and contaminated laboratory instruments, solutions, and glassware. The following procedures should be followed to limit RNase contamination when working with RNA:

  • Always wear gloves while working and change gloves frequently
  • Refrain from using reagents, consumables and equipment that are in common use for other general lab processes
  • Use dedicated RNase free equipment such as pipettes, pipette tips, gels boxes, etc.
  • Work in a separate room, fume hood or lab space if available
  • Use plastic, disposable consumables that are certified RNase free
  • Purchase reagents, such as commonly used buffers and water, that are certified RNase free. Prepare small individual aliquots of such buffers to avoid repeated transfer out of stock buffers. This lowers the risk of contamination of the stock solution
  • Wipe down work surfaces with commercial RNase inhibiting surfactant solutions or 70% ethanol before starting work
  • Keep the RNA on ice after extraction and while working with it.
  • Store the extracted RNA at -20°C. For long term stability, keep the RNA at -80°C.
Don't miss out!
Subscribe To Newsletter
Receive top education news, lesson ideas, teaching tips and more!
Invalid email address
Give it a try. You can unsubscribe at any time.

Certification of Analysis (COA)

Lot No. R01944748           Lot No. R01944846

Don't miss out!
Subscribe To Newsletter
Receive top education news, lesson ideas, teaching tips and more!
Invalid email address
Give it a try. You can unsubscribe at any time.