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NGS Size Selection Kits: Everything You Need to Know

Sep 2, 2025 AMD Biotech Inc

When it comes to Next-Generation Sequencing (NGS), sample quality is everything. From maximizing library yields to removing unwanted fragments, DNA size selection is a critical step that directly impacts sequencing accuracy and efficiency.

At AMD Biotech, we understand that researchers need reliable, consistent, and high-performance tools to prepare their samples. That’s why we offer a range of Auto-Mag® Size Selection Kits tailored for diverse workflows — from high molecular weight DNA extraction to precise library fragment selection.

Why DNA Size Selection Matters

During library preparation, DNA fragments of the wrong size can affect sequencing results, increase costs, and lead to wasted reads. By using magnetic bead-based size selection, you can:

  • Remove small contaminants like primer dimers and adapter dimers
  • Eliminate overly large fragments that could skew data
  • Enrich for the target size range to maximize sequencing efficiency

Our Size Selection Solutions

We’ve designed our kits to work seamlessly in your existing workflows, with no protocol changes needed from other commonly used beads.

1. Auto-Mag® X-Pure Size Select NGS

  • For: Versatile library size selection and cleanup
  • Advantages:
    • Works across a broad range of fragment sizes (100 bp to >1 kb)
    • High recovery rates
    • Compatible with Illumina®, Oxford Nanopore®, and other platforms
  • Applications: Post-PCR cleanup, fragment selection before sequencing

2. Auto-Mag® HMW Size Selection

  • For: Long-read sequencing workflows and high molecular weight DNA
  • Advantages:
    • Preserves large DNA fragments for PacBio® and Nanopore® sequencing
    • Ideal for genomic assemblies and structural variant analysis
  • Applications: Long-read NGS prep, de novo sequencing projects

Tips for Best Results

  • Mix thoroughly: Vortex bead suspension before use and pipette mix or vortex with the sample to ensure complete DNA binding.
  • Optimize bead-to-sample ratios: Adjust ratios depending on your target size range.
  • Elute in the right buffer: Low-salt, low-EDTA buffers work best for downstream applications.

Why Choose AMD Biotech Beads?

  • ISO 9001:2015 certified manufacturing in Atlanta, GA
  • Consistent performance batch-to-batch
  • More Cost-effective compared to other similar brand products

 

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