Auto-Mag® DTR

Auto-Mag® DTR utilizes AMD’s proprietary magnetic bead technology and is a high-performance reagent designed for the efficient removal of BigDye terminators and sequencing reaction contaminants from Sanger sequencing reactions, ensuring reliable and high-quality DNA sequence reads.

The Auto-Mag® DTR workflow involves selective binding of DNA to paramagnetic beads, followed by the removal of excess nucleotides, primers, and non-target amplicons through washing steps, and concluding with the elution of purified DNA.

Compatible with both manual and fully automated workflows, Auto-Mag® DTR provides a flexible and reliable solution for sequencing reaction cleanup. The protocol is readily adaptable to common liquid handling systems, supporting high-throughput processing.

Key Features

• Efficient Removal of BigDye Terminators
  Effectively removes unincorporated dye terminators and sequencing contaminants
• Improved Sequencing Quality
  Enhances signal clarity and enables longer, high-quality read lengths
• Magnetic Bead-Based Purification
  Streamlined workflow with selective DNA binding, washing, and elution
• Reliable Contaminant Removal
  Eliminates excess nucleotides, primers, salts, and non-target amplicons
• Automation-Ready
  Compatible with common liquid handling systems for high-throughput processing
• Flexible Workflow Integration
  Suitable for both manual and fully automated sequencing cleanup workflows
• Cost-Effective Alternative
  Provides consistent performance without the need for precipitation or column-based methods

Product Contents

Auto-Mag® DTR provides the reagents required for magnetic bead–based purification of Sanger sequencing reactions.

Sanger Sequencing Cleanup Workflow

1. Confirm the volume of Sanger sequencing reaction samples.
2. Add 10μl of Auto-Mag® DTR and the corresponding volume of 85% of Ethanol for DNA binding.
3. Separate beads from contaminants.
4. Wash beads/DNA twice with 85% of Ethanol to remove contaminants.
5. Elute purified DNA from beads.
6. Transfer eluate to new vessel and ready for sequencing. 

Data & References 

1. Comparison of Sanger Sequencing Clean-Up Reagents

Fig 1. The 1.4 kb PCR fragments were sequenced using Applied Biosystems’ BigDye Terminator v3.1 Chemistry and cleanup with Auto-Mag® DTR, or other company's sequencing cleanup reagent for comparison.  the DNA samples were analyzed on an Applied Biosystems’ 3730XL.

1. The sequencing chromatogram 1:  the samples purified with Auto-Mag® DTR.
2. The sequencing chromatogram 2: the sample purified with company A sequencing cleanup reagent.
3. The sequencing chromatogram 3: the unpurified samples.

No dye blobs or ski slope effects were observed in sequencing samples purified using Auto-Mag® DTR or Company A’s cleanup reagents, in contrast to unpurified samples.

2. Contiguous read length (CRL) of sequencing sample after cleanup with Auto-Mag® DTR

Fig 2. A 10 µl sequencing reaction sample was purified using 10 µl of Auto-Mag® DTR. The DNA was analyzed on an Applied Biosystems 3730XL sequencer. No dye blobs were observed, and the Contiguous Read Length (CRL) exceeded 1000 bp.