Auto-Mag® Plant DNA Plus Kit

Plant DNA Isolation Kit

Isolate purified, high-quality, and reliable gDNA rapidly from various plant and fungi species. Use purified gDNA for downstream and biotech applications with the quick technique. The kit contains a manual and guidelines to help it adapt to current liquid handling workstations in the marketplace.

The Plant DNA Isolation kit is highly efficient and can produce a significant amount of purified gDNA from 50mg of wet tissue or 30mg of dry tissue. Our specially manufactured magnetic particles bind to the gDNA particles specifically while the contamination remains. 

Downstream Applications That Require The Plant DNA Isolation Kit 

• Enzyme Digestion PCR, 
• Next Generation Sequencing, 
• Southern Blot, 
• Chip Detection, 
• Hybridization Applications etc. 

Our Plant DNA Isolation kit consists of two additional lysis enhancers, PLE-1 (SDS lysis method) or PLE-2 (CTAB lysis method). A user can combine the enhancer with the PDL (Plant DNA Lysis Buffer) considering the plant tissue because several polysaccharide polyphenols-rich plant tissues do not lyse well in SDS-based lysis buffer. 

The yield of gDNA from the Plant DNA Isolation kit varies with plant species, tissues, genome size, ploidy, cell number, sample age, and storage conditions. 

Auto-Mag® Plant DNA Plus Kit uses Auto-Mag® magnetic beads technology and is specially designed for rapid and reliable purification of gDNA from a wide range of plant and fungi species. The procedures of the Auto-Mag® Plant DNA Isolation Kit describe the isolation of DNA from 50 mg samples of wet tissue (or 30 mg dry tissue). Under DNA binding conditions only the genomic DNA will bind to the magnetic particles while most of the contamination is removed. Purified genomic DNA is suitable for various conventional operations, including enzyme digestion, PCR, Next Generation Sequencing, Southern blot, chip detection, hybridization applications and other experiments. The protocol can be used for manual procedures as well as guidelines for adapting it to automatic liquid handling workstations currently on the market.

DNA yield varies between different species and tissues and depends on genome size, ploidy, cell number, age of tissue sample, and storage condition of the sample. Some plant tissues rich in polysaccharide polyphenols do not lyse well in SDS-based lysis buffer. This kit includes two additional plant lysis enhancers: PLE-1(based on SDS lysis method) or PLE-2, (based on CTAB lysis method). User can choose either one to combine with Plant DNA Lysis Buffer (PDL) for different plant species and tissues lysis.

Highlights & Applications

Highlights

• Fast and easy processing using a magnetic bead system
• Purified PCR grade DNA with no inhibiting substances (polysaccharides and phenols)
• Highly specific binding of DNA allows easy removal of impurities and inhibitors
• Additional plant lysis enhancers can work for different plant species and tissues
• Automation-friendly
• Eluted DNA is well suitable for most following applications..

Application

Plant genomic DNA isolation for:

• PCR, Real-time PCR
• Cloning, genotyping
• Sequencing
• Plant breeding

Product Documents

Auto-Mag® Plant DNA Isolation Kit – Protocol

Auto-Mag® Plant DNA isolation Kit – MSDS

Additional Information

Certification of Analysis (COA)