Auto-Mag® FFPE Tissue DNA Isolation Kit

Auto-Mag® FFPE Tissue DNA Isolation Kit utilizes the paramagnetic bead-based technology and is specially designed to isolate DNA from formalin-fixed, paraffin-embedded (FFPE) tissue without the use of xylene. Auto-Mag® FFPE Tissue DNA Isolation Kit provides a special deparaffinization reagents and compatible extraction buffer for DNA isolation.  This combination allows the entire process of isolation to be performed in the same tube, minimizing sample loss and cross-contamination. Purified DNA is suitable for a variety of downstream applications, such as: Targeted amplicon NGS, Targeted capture NGS, Whole exome sequencing, and Whole genome sequencing, Endpoint, or qPCR, etc.

The isolation can be performed in both 96-well plates (manually and automated) and in 1.5 mL tubes (manually only) with related protocols.  Nucleic acid extraction begins with the solubilization of the paraffin from the tissue slices first. An enzymatic lysis step digests the tissue and releases the nucleic acids, followed by decrosslinking at a high temperature.  The binding solution is added to immobilize the nucleic acids to the surface of the Auto-Mag® D-3 beads. Contaminants are rinsed away using a simple washing procedure and the nucleic acids are eluted with elution buffer.

Highlights & Applications

Highlights

• No Xylene – avoid toxic chemicals
• No need to transfer samples – minimize sample loss
• Beads compatible with deparaffinization reagent – single tube/plate processing
• Highly specific binding of DNA allows easy removal of impurities and inhibitors
• Automation-friendly
• Eluted DNA is well suitable for most following processing

Application

Genomic DNA isolation from FFPE tissues for:

• PCR, Real-time PCR
• Cloning, genotyping
• Sequencing
• Cancer and Alzheimer’s disease research

Product Documents

Auto-Mag® FFPE Tissue DNA Isolation Kit – Protocol

Auto-Mag® FFPE Tissue DNA Isolation Kit – MSDS

Additional Information

Summary of DNA yield and purity for FFPE tissue samples using AMD Auto-Mag® FFPE Tissue DNA Isolation Kit

Certification of Analysis (COA)

Lot No. D00844208           Lot No. D00844890