$0.00 – $728.00
Xylene-free, Magnetic bead-based reagent for automated or manual isolation of DNA from FFPE tissue samples
Auto-Mag® FFPE Tissue DNA Isolation Kit utilizes the paramagnetic bead-based technology and is specially designed to isolate DNA from formalin-fixed, paraffin-embedded (FFPE) tissue without the use of xylene. The Kit provides a special deparaffinization reagents and compatible extraction buffer for DNA isolation. This combination allows the entire process of isolation to be performed in the same tube, minimizing sample loss and cross-contamination. The purified DNA is of high yield and integrity and is free of inhibitors, ready for use in several downstream applications including qPCR, mutation screening, microarray analysis, sequencing, single nucleotide polymorphism (SNP) and short-tandem repeat (STR) genotyping, etc.
The isolation can be performed in both 96-well plates (manually and automated) and in 1.5-2.0 ml tubes (manually only) with related protocols. Nucleic acid extraction begins with the solubilization of the paraffin from the tissue slices first. An enzymatic lysis step digests the tissue and releases the nucleic acids, followed by decrosslinking at a high temperature. The binding solution is added to immobilize the nucleic acids to the surface of the Auto-Mag® D-3 beads. Contaminants are rinsed away using a simple washing procedure and the nucleic acids are eluted with elution buffer.
Features
Auto-Mag® FFPE Tissue DNA Isolation Kit Protocol V.2.1
Auto-Mag® FFPE Tissue DNA Isolation Kit – MSDS