Auto-Mag® Viral RNA / DNA Isolation Kit

Auto-Mag® Viral RNA/DNA Isolation Kit is designed to use magnetic bead-based technology to purify high-quality nucleic acid from whole blood, serum, plasma, swabs, saliva, and other bodily fluids. The isolation kit combines the enhanced lysis/binding buffer formulation and reversible nucleic acid-binding properties of Auto-Mag® magnetic particles that provides the highest sensitivity in viral RNA/DNA recovery.  The viral RNA or DNA extraction can be performed manually or on automated platforms with equivalent results. The isolated viral RNA or DNA can be directly used in downstream applications without the need for further purification.

Highlights & Applications

Highlights

• Magnetic bead-based chemistry with no centrifugation or filtration
• High yield and quality viral RNA/DNA Isolation
• Adaptable to various automated liquid handling workstations
• No toxic organic solvents
• Available in a 96-well format that can be integrated with a robotic automation system

Application

 Total Viral RNA DNA isolation for:

• PCR, RT-qPCR, RT-PCR
• Virus detection
• Sequencing

Product Documents

Auto-Mag® Plasmid DNA Miniprep Kit – Protocol

Auto-Mag® Plasmid DNA Miniprep Kit – MSDS

Additional Information

Working in RNase Free Conditions

RNA purity and integrity is essential for downstream applications. RNA can be degraded by RNase A, which is a highly stable contaminant found in any laboratory environment. Some general precautions should be followed to avoid the introduction of contaminating nucleases especially during wash and elution steps. The most common sources of RNase contamination are hands, dust particles, and contaminated laboratory instruments, solutions, and glassware. The following procedures should be followed to limit RNase contamination when working with RNA:

• Always wear gloves while working and change gloves frequently
• Refrain from using reagents, consumables and equipment that are in common use for other general lab processes
• Use dedicated RNase free equipment such as pipettes, pipette tips, gels boxes, etc.
• Work in a separate room, fume hood or lab space if available
• Use plastic, disposable consumables that are certified RNase free
• Purchase reagents, such as commonly used buffers and water, that are certified RNase free. Prepare small individual aliquots of such buffers to avoid repeated transfer out of stock buffers. This lowers the risk of contamination of the stock solution
• Wipe down work surfaces with commercial RNase inhibiting surfactant solutions or 70% ethanol before starting work
• Keep the RNA on ice after extraction and while working with it.
• Store the extracted RNA at -20°C. For long term stability, keep the RNA at -80°C.

Certification of Analysis (COA)