Proteinase K, recombinant (20mg/ml)

Proteinase K is a nonspecific serine protease that is useful for the general digestion of proteins. Proteinase K cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic, or hydrophobic amino acids. The smallest peptide to be hydrolyzed by this enzyme is tetrapeptide. The molecular weight of Proteinase K is 28,900 Daltons (28.9 kDa). This enzyme exhibits high stability and activity in the presence of SDS, EDTA, and urea, as well as over a wide pH range. Proteinase K is used for the destruction of proteins in cell lysates (tissue, cell culture cells) and for the release of nucleic acids. Proteinase K is useful for the inactivation of nucleases and lysis during the isolation of DNA and RNA.

Features

• Stable at Room Temperature and Easy to Use
• High activity in elevated temperatures (up to 56°C) and under denaturing conditions
• Stable over a wide pH range of 4.0–12.5 for use in a variety of applications

Applications

• Isolation of plasmid and genomic DNA
• Isolation of genomic DNA from mouse tail
• Isolation of genomic DNA from cultured cells
• Isolation of RNA
• Inactivation of RNases, DNases and enzymes in reactions
• Improving cloning efficiency of PCR products.

The proteinase K solution has been sterile filtered, which can maintain its activity for a long time at room temperature and low temperature. It is recommended to store at 2-8 ℃ for long-term storage.

Product Data & Figures

Product Documents

Proteinase K, recombinant (20mg/ml)

Proteinase K Solution MSDS

Certification of Analysis (COA)

Lot # B30145383

Frequently asked questions (FAQs)