Auto-Mag® HMW Size Select (Mega Select)

Auto-Mag® HMW Size Select consists of AMD Biotech's own paramagnetic beads and compatible buffers. By simply adjusting the volume ratio of Auto-Mag® HMW Size Select to sample, DNA fragments: <5kb, <10kb, or <20kb in length can be quickly removed to trace levels from high molecular weight (HMW) DNA samples and desired large size DNA are recovered, which leads to significantly enhanced mean read length of third-generation sequencing processes, such as PacBio SMRT Technology or Oxford Nanopore Technology.

• Works with HMW DNA
• Effective purification and recovery of HMW DNA, dsDNA fragments 5kb or longer
• Removal of short DNA fragments, impurities, and unwanted reaction components
• Fragment size selection of HMW DNA for specific applications of long-read sequencing.
• Expectant recover Yield: Up to 90%
• Compatible with manual and automated processing and cost effective.

The Auto-Mag® HMW Size Select Kit can be used to quickly cleanup unsheared HMW DNA samples and can also be used to selectively recover desired size HMW DNA fragments from fragmented HMW DNA samples or even partially degraded gDNA samples for library preparation of long-read sequencing (LRS). Since Auto-Mag® HMW Size Select uses magnetic technology and requires no centrifugation or filtration, it is suitable for manual processing as well as adapting to automatic liquid handling workstations.

Product name change notice: To intuitively clarify the characteristics and functions of this product, the name of Auto-Mag® Mega Select  is changed to Auto-Mag® HMW Size Select. There are no changes in the characteristics, quality and usage of the product.

HMW gDNA Size Selection & Cleanup Workflow

1.  Confirm the gDNA sample volume.
2.  Add appropriate volume of Auto-Mag® HMW Size Select  to the sample.
3.  Rotate the sample at room temperature for 10 minutes for selective binding HMW DNA
4.  Separate the beads from contaminants and short DNA fragments.
5.  Wash magnetic beads with 85% ethanol.
6.  Elute size-selective HMW gDNA from magnetic beads.

Data & References

The HMW DNA size selection and cleanup

Fig 1. The different size range of short DNA fragment were deleted by using 25µl of gDNA mixture at different ratios of Auto-Mag® HMW Size Select volume to gDNA sample volume. Recovered HMW DNA were eluted in 25µl and analyzed on an Agilent TapeStation 2200.

Comparison of <10kb DNA deletion

Fig 2. Comparison of HMW DNA selection on gDNA spiked with 1kb Plus DNA ladder at 50ng/μl using Auto-Mag® HWM Size Select (1.5x) and AMPure PB (0.45x) according to their respective protocol. Data were analyzed on an Agilent TapeStation 2200 system. Auto-Mag® HMW Size Select has better performance in removing <10kb DNA fragments.