Auto-Mag® Carboxyl NanoBeads (C-9)
Product Information
Highlights
- Designed to use for nucleic acid (DNA/RNA) fragment size selection, purification, and recovery
- Compatible with automated platform
- Narrow size distribution, CV≤5%
- Extremely stable in organic solvents and aqueous buffers.
Auto-Mag® Carboxyl Nanobeads (C-9) are carboxyl-functionalized, monodisperse, superparamagnetic particles featuring rapid magnetic response, high binding capacity, excellent sensitivity, and superior physical stability. Supplied as a 5% bead suspension, Auto-Mag® Carboxyl Nanobeads (C-9) can be formulated with PEG 8000 and NaCl-based buffer systems to enable selective binding, purification, and recovery of DNA fragments based on size. With excellent performance, consistent batch-to-batch quality, and cost-effectiveness, Auto-Mag® Carboxyl Nanobeads (C-9) provide a versatile platform for NGS library preparation, PCR product cleanup, DNA/RNA purification, size selection, and post-enzymatic reaction cleanup, supporting reliable and reproducible results across molecular biology workflows.
Auto-Mag® Carboxyl NanoBeads (C-9) deliver superior yield, purity, and quality compared to leading competitors. Their unmatched value stems from in-house design and manufacturing, ensuring stringent quality control, enhanced performance, and cost efficiency.
Application
Auto-Mag® Carboxyl NanoBeads (C-9) offer broad application versatility and are ideal for developing reagents for nucleic acid (DNA/RNA) purification, cleanup, recovery, and fragment size selection.
- Sanger sequencing dye terminate removing,
- PCR amplicon clean-up,
- DNA fragment size selection,
- NGS library purification,
Note: To more clearly identify the surface functional groups of the magnetic beads, Auto-Mag® NanoBeads (C-9) have been renamed Auto-Mag® Carboxyl NanoBeads (C-9)
Workflow of DNA or PCR cleanup
The below illustrates the workflow for purifying PCR products using a DNA/PCR purification and recovery reagent formulated with Auto-Mag® Carboxyl NanoBeads (C-9).
- Bind DNA to magnetic beads using a 1.8× reagent-to-sample volume ratio.
- Magnetically separate the DNA-bound beads from contaminants.
- Wash the magnetic beads with 70% ethanol to remove residual contaminants.
- Elute the purified DNA from the magnetic beads.
- Transfer the purified DNA to a new storage vessel.
Data & References
Figures 1 and 2 below demonstrate PCR product purification and recovery using Auto-Mag® PCR-Pure, a DNA/PCR purification and recovery reagent formulated with Auto-Mag® Carboxyl NanoBeads (C-9), and compare its purification and recovery performance with that of comparable commercial products.
PCR amplicons purified using Auto-Mag® PCR-Pure with 1.8x ratio.
Fig 1. Demonstrate that the PCR amplicons purified using Auto-Mag® PCR-Pure with 1.8x ratio. The purified PCR products were analyzed on the 1.2% agarose gel electrophoresis which shows that Auto-Mag® PCR-Pure reagent provides predictable performance while maintaining efficient recovery.
Comparison of purification and size selection
Fig 2. Comparison of purification and size selection on 20μl of DNA ladder mix at 1.8x ratio set using Auto-Mag® PCR-Pure reagent and AMPure XP reagents following manufacturer’s recommended protocols. The purified DNA was eluted in 20μl and analyzed on Agilent's Tapestation® 2200. The results show that both reagents provide predictable performance while maintaining efficient and similar recovery.
DNA size selection and Cleanup
Figures 3 and 4 below demonstrate DNA fragment size selection and recovery using Auto-Mag® X-Pure Size Select, formulated with Auto-Mag® Carboxyl NanoBeads (C-9), and compare its DNA size-selection performance with that of comparable commercial products.
DNA size selection
Fig 3: DNA size selection was performed using 20 µl of GeneRuler Ultra Low Range DNA Ladder and 100 bp DNA Ladder mix at different ratios of Auto-Mag® X-Pure Size Select volume to DNA sample volume (1.8x to 0.4x). Selected DNA fragments were eluted in 20 µl and analyzed on an Agilent TapeStation 2200.
Comparison of Double-Sided Size Selection
Fig 4. Comparison of electropherograms of the double-sided size selection on sheared gDNA at 0.7x/0.2x ratio set using Auto-Mag® X-Pure Size Select and a similar kit from Company A following the manufacturer’s recommended protocol. DNA was eluted in 25 µL and analyzed on an Agilent TapeStation® 2200.
Total RNA Cleanup & Purification
Figure 5 below illustrates total RNA cleanup and recovery using Auto-Mag® RNA-Pure, an RNA/cDNA purification and size-selection reagent formulated with Auto-Mag® Carboxyl NanoBeads (C-9).
Fig 5: The 20 µl of RNA at 20 ng/µl, 10ng/µl, and 5ng/µl was cleaned up with Auto-Mag® RNA-Pure reagent following recommended protocols. The RNA was eluted in 20 µl and analyzed on Agilent’s TapeStation® 2200. Recovery rates ranged over 85% respectively.
Specifications
- Technology:
- Paramagnetic bead-based technology
- Ligand
- Carboxylate modified particles
- Form: :
- Suspension (5% Solids)
- Concentration: :
- 50 mg/ml
- Average Particle Size:
- ~1 µm.
- Application:
- DNA/RNA size select, cleanup & recovery
- Shipping/Storage:
- Shipping at ambient temperature/Store at 2–8°C.
- ============================