Auto-Mag® Quantitative Normalized Plasmid DNA Preparation Kit

Auto-Mag® Quantitative Normalized Plasmid DNA Preparation Kit combines classic alkaline SDS lysis, an endotoxin removal washing system, and AMD’s proprietary superparamagnetic bead DNA normalization technology for rapid, easy, and direct quantitative isolation of high-quality, endotoxin-free (<0.1 EU/µg) plasmid DNA from bacterial cultures.

Designed for simplicity and efficiency, the standard procedure yields approximately 2 µg of plasmid DNA (2–10 kb) per preparation with low variation between samples and performs reliably even with large sample volumes. Its streamlined workflow minimizes manual handling time and reduces experimental errors, making it ideal for high-throughput laboratories and routine molecular biology workflows. The purified, normalized plasmid DNA is suitable for downstream applications such as cloning, sequencing, transfection, and research in biomedicine, gene expression, antibiotic resistance, and gene therapy development.

Customized optional protocols accommodate different DNA recovery needs, including plasmids larger than 10 kb. The kit supports both magnetic bead–based and centrifugation-based purification workflows, compatible with manual and automated platforms. For more information, please contact AMD Biotech.

Key Features:

• All-in-One Workflow – Combines plasmid extraction and DNA normalization in a single process.
• Consistent, Quantitative Yields – Standardized recovery of ~2 µg plasmid DNA per sample.
• High Purity and Quality – Produces endotoxin-free (<0.1 EU/µg) plasmid DNA suitable for sensitive downstream applications.
• Scalable and Reproducible – Maintains efficiency and reliability across large sample batches.
• Flexible Operation – Compatible with both magnetic bead–based and centrifugation-based workflows.
• Automation Ready – Easily adapted for high-throughput or automated platforms.

Overview of the Workflow for Normalized Plasmid DNA Isolation

• Prepare bacterial cultures and pellet the cells by centrifugation.
• Lyse the cells using alkaline lysis buffer.
• Remove cellular debris and genomic DNA by centrifugation.
• Bind plasmid DNA with magnetic beads and normalization buffer.
• Separate beads magnetically and wash.
• Elute the normalized plasmid DNA.

Data & References

Normalized Plasmid DNA Isolation

Figure 1. Plasmid extraction was performed using an E. coli strain carrying the pUC19 plasmid cultured at 37°C for 16 hours to an OD₆₀₀ of 2.9. Different culture volumes (0.5–2 mL) were processed either with the standard plasmid extraction protocol (samples 1, 3, 5, and 7) or with the 2 µg quantitative normalized plasmid DNA preparation protocol (samples 2, 4, 6, and 8). Plasmid DNA was eluted in 100 µL of Elution buffer, analyzed by 1.2% agarose gel electrophoresis, quantified using the Invitrogen Qubit assay and Agilent TapeStation 2200, and assessed for residual endotoxin using a Chromogenic LAL Endotoxin Assay Kit.

The results show that control samples yielded variable amounts of plasmid DNA, ranging from 6.8 µg to 2.8 µg depending on the culture volume. In contrast, samples processed using the normalized plasmid DNA preparation protocol consistently produced approximately 2 µg of plasmid DNA across the tested culture volume range (0.5–2 mL). These results demonstrate that the Quantitatively Normalized Plasmid DNA Prep Kit reliably delivers high-quality plasmid DNA with consistent yield, independent of the starting culture volume.