Auto-Mag® X-Pure Size Select

• Cost-effective alternative to benchmark solutions

• Fast and reliable post-PCR and DNA cleanup reagent

• High recovery of amplicons >100 bp

• Single- or double-sided size selection for NGS with uniform fragment size distribution

• Flexible sample volume compatibility with scalable performance

• Lot-to-lot consistency for reproducible results

• Compatible with both manual and automated workflows

Auto-Mag® X-Pure Size Select utilizes advanced paramagnetic bead technology for efficient DNA cleanup across various genomic applications, including sequencing, qPCR/ddPCR/PCR, microarrays, and enzymatic reactions.

For PCR product purification, its optimized buffer system selectively binds DNA fragments larger than 100 bp, effectively removing primers, nucleotides, salts, and enzymes through three simple steps: binding, washing, and elution.

In DNA size selection, researchers can precisely control fragment recovery by adjusting the reagent-to-sample volume ratio, ensuring accurate and reproducible results.

Designed for both manual and automated workflows, Auto-Mag® X-Pure Size Select offers a cost-effective, high-performance solution for DNA purification and cleanup, meeting the stringent demands of advanced sequencing applications.

DNA Cleanup & Size Select Workflow

PCR Cleanrup, Left Side Size Selection

1. Bind PCR amplicon or ds-DNA to magnetic beads.
2. Separate beads from contaminants.
3. Wash magnetic beads with 85% ethanol to remove contaminants.
4. Elute DNA from magnetic beads.
5. Transfer purified DNA to new storage vessels.

 Double Side Size Selection 

1. Confirm the sample volume.
2. Add corresponding volume of Auto-Mag® X-Pure Size Select to the sample for first binding.
3. Separate the beads and transfer supernatant to a new vessels.
4. Add corresponding volume Auto-Mag® X-Pure Size Select to the supernatant for second binding.
5. Separate beads from contaminants.
6. Wash magnetic beads with 85% ethanol to remove contaminants.
7. Elute DNA from magnetic beads.
8. Transfer to new vessels.

Data & References

DNA size selection and Cleanup

Fig 1: DNA size selection was performed using 20 µl of GeneRuler Ultra Low Range DNA Ladder and 100 bp DNA Ladder mix at different ratios of Auto-Mag® X-Pure Size Select NGS volume to DNA sample volume (1.8x to 0.4x). Selected DNA fragments were eluted in 20 µl and analyzed on an Agilent TapeStation 2200.

Comparison of Double-Sided Size Selection

Fig 2. Comparison of electropherograms of  the double-sided size selection on sheared gDNA at 0.7x/0.2x ratio set using Auto-Mag® X-Pure Size Select (NGS) and a similar kit from Company A following the manufacturer’s recommended protocol. DNA was eluted in 25 µL and analyzed on an Agilent TapeStation® 2200.