Auto-Mag® X-Pure Size Select

• Designed direct, cost-efficient alternative to the Benchmark
• Rapid and reliable post-PCR and DNA clean-up reagent
• High recovery of amplicons >100 bp
• Single, or double-sided size selection capability with uniform fragments size distribution
• Rapid and reliable clean-up total RNA, including miRNA, siRNA, and aRNA
• Flexibility of starting sample volumes with the ability to scale and consistent results
• Compatible with manual and automated processing and cost-effective

Auto-Mag® X-Pure Size Select reagnet employs advanced paramagnetic bead technology for efficient PCR amplicon clean-up, DNA fragment and RNA purification, and precise DNA size selection during next-generation sequencing (NGS) library construction. With a seamless “bottle swap” feature, it offers hassle-free adoption with no protocol changes compared to major competitors.

For PCR product purification, the reagent supports both single-tube and high-throughput 96/384-well formats. The optimized buffer system binds DNA fragments >100 bp, effectively removing primers, nucleotides, salts, and enzymes through a simple wash process. Purified DNA is eluted in low-salt buffer or water, ready for downstream applications.

During DNA size selection, researchers can fine-tune fragment recovery by adjusting the reagent-to-sample volume ratio, achieving precise and uniform results with three straightforward steps: binding, washing, and elution.

This RNase-free reagents also excel in RNA cleanup and concentration, supporting applications like miRNA, siRNA, aRNA purification, and RNA-Seq library preparation. Its flexibility accommodates various sample sizes, ensuring scalability and consistent results.

Auto-Mag® X-Pure Size Select is compatible with both manual and automated workflows, offering a cost-effective, reliable, and high-performance solution for DNA and RNA purification in even the most demanding sequencing applications.

Product name change notice: To intuitively clarify the characteristics and functions of this product, the name of Auto-Mag® X-Pure Select  is changed to Auto-Mag® X-Pure Size Select. There are no changes in the characteristics, quality and usage of the product.

DNA Size Select Workflow

PCR Cleanrup, Left Side Size Selection

1. Bind PCR amplicon or ds-DNA to magnetic beads.
2. Separate beads from contaminants.
3. Wash magnetic beads with 85% ethanol to remove contaminants.
4. Elute DNA from magnetic beads.
5. Transfer purified DNA to new storage vessels.

 Double Side Size Selection 

1. Confirm the sample volume.
2. Add corresponding volume of Auto-Mag® X-Pure Size Select to the sample for first binding.
3. Separate the beads and transfer supernatant to a new vessels.
4. Add corresponding volume Auto-Mag® X-Pure Size Select to the supernatant for second binding.
5. Separate beads from contaminants.
6. Wash magnetic beads with 85% ethanol to remove contaminants.
7. Elute DNA from magnetic beads.
8. Transfer to new vessels.

Data & References

DNA size selection and Cleanup

Fig 1: DNA size selection was performed using 20 µl of GeneRuler Ultra Low Range DNA Ladder and 100 bp DNA Ladder mix at different ratios of Auto-Mag® X-Pure Size Select NGS volume to DNA sample volume (1.8x to 0.4x). Selected DNA fragments were eluted in 20 µl and analyzed on an Agilent TapeStation 2200.

Comparison of Double-Sided Size Selection

Fig 2. Comparison of electropherograms of  the double-sided size selection on sheared gDNA at 0.7x/0.2x ratio set using Auto-Mag® X-Pure Size Select (NGS) and a similar kit from Company A following the manufacturer’s recommended protocol. DNA was eluted in 25 µL and analyzed on an Agilent TapeStation® 2200.

Total RNA Clean-Up

Fig 3. The 20μl of total RNA at 300 ng/μl  were cleaned up with Auto-Mag® X-Pure Size Select following recommended protocols. The purified RNA was eluted in 50μl and analyzed on Agilent’s TapeStation® 2200. Recovery rates ranged between 90-95% respectively