Auto-Mag® Yeast DNA Miniprep Kit ( with 2 ml Prefilled Disruptor Tubes)
Product Information
- Rapid and convenient method to isolate genomic DNA from (up to 1 x 108) yeast and fungus species
- Yields high quality DNA that is ready for PCR and other downstream applications.
- Recovery up to 10µg of genomic DNA from 1 x 108 yeast cells.
- Automation-friendly and can be easily scaled up for high throughput.
Auto-Mag® Yeast DNA Miniprep Kit utilizes advanced magnetic bead technology for the rapid and reliable isolation of high-quality total cellular DNA from various yeast species. The kit efficiently processes up to 1.5 mL of log-phase yeast culture (OD600 of 1.0 in YPD medium), making it ideal for streamlined and reproducible DNA extraction workflows.
Auto-Mag® Yeast DNA Miniprep Kit workflow begins by adding lysis buffer to the yeast sample and transferring the mixture to a disruptor tube. The sample is then mixed and heated for 5 minutes. Following this, the sample is homogenized for 5 minutes using an appropriate disruptor to ensure efficient cell lysis. Next, digestion enzymes are added to the sample, which is incubated at 55°C for 20 minutes to release the DNA effectively. The sample is then centrifuged, and the supernatant is transferred to a DNase-free microcentrifuge tube. For RNA-free genomic DNA, an optional RNase treatment can be performed at this stage. DNA is captured using magnetic beads in the presence of a binding buffer. After two quick wash steps to remove salts and protein contaminants, the purified DNA is eluted with an elution buffer. The resulting high-quality DNA is suitable for downstream applications such as PCR, restriction digestion, and hybridization techniques.
The Auto-Mag® Yeast DNA Miniprep Kit use magnetic bead technology, making it suitable for both manual processing and seamless adaptation to automated liquid handling workstations. For assistance with automation integration, please contact us for dedicated support.
Yeast DNA Isolation Workflow
- Prepare yeast cultures & pellet the cells.
- Add disrupt lysis buffers and homogenize cells.
- Add beads to bind nucleic acids.
- Separate the beads from contaminants
- Wash magnetic to remove contaminants.
- Elute DNA from magnetic beads.
Specifications
- Isolation Technology: Magnetic Beads
- Sample Sources:Yeast
- Sample Volume:≤ 1.5 ml of Culture
- Typical Yield: Dependent upon sample,
- Application:PCR, restriction digestion, and hybridization techniques. NGS.
- Shipping Ambient temperature.
- Shelf life: 12 months from date of delivery & 24 months from date of production.