Auto-Mag® Blood & Tissue DNA Isolation Kit

Auto-Mag® Blood & Tissue DNA Isolation Kit uses magnetic bead-based workflows and combines multiple sample processing steps together for high quality genomic DNA purification from various sources including fresh or frozen whole blood, buffy coat, saliva, fresh or frozen mammalian tissues and cells lines.

• Fast and easy processing using a magnetic bead system.
• Isolate high-quality Genomic DNA from various sources samples.
• No organic extraction or alcohol precipitation
• Complete removal of contaminants and inhibitors
• Additional Automation-ready protocols for IsoPure Mini, IsoPure 96, NAP-16 or KingFisher system, etc.

Up to 96 samples of tissues can be processed in less than an hour. Purified high-quality genomic DNA is suitable for direct use in most common molecular biology applications: PCR, conventional restriction digestion, cloning, DNA sequencing, and Southern blot analysis.

Auto-Mag® Blood & Tissue DNA Isolation Kit uses a simple 4 step procedure: Lyse-Bind-Wash-Elute. Follow the instructions, the extraction can be performed manually or on automated platforms with equivalent results.

Manual Workflow for Blood DNA isolation

1. Confirm the sample volume.
2. Add Proteinase K to lysis the cells.
3. Add Beads to bind DNA
4. Separate beads from contaminants.
5. Wash magnetic beads to remove contaminants.
6. Elute DNA from magnetic beads

Data & References

Comparison of DNA Isolated from Whole Blood

Figure 1. Comparison of DNA Isolated from Whole Blood. gDNA was isolated from 50, 100 and 200 µL of whole blood using Auto-Mag® Blood & Tissue DNA Isolation Kit and a competitor’s M kit. Following isolation, 10 µL from each 100 µL elution was loaded on 1.2 % agarose gel, with the results indicating that the DNA isolation was consistent between the samples .  In terms of determined recovery, Auto-Mag® Blood & Tissue DNA Isolation  Kit shows a higher recovery than the competitor’s kit.