Auto-Mag® Blood & Tissue DNA Isolation Kit
Product Information
Auto-Mag® Blood & Tissue DNA Isolation Kit provides a simple and reliable solution for genomic DNA purification from whole blood, cultured cells, and a broad range of animal tissues. The optimized magnetic bead–based chemistry combines efficient sample lysis, selective DNA capture, rapid contaminant removal, and convenient elution in a streamlined workflow. Following sample lysis and protein digestion, genomic DNA is captured onto magnetic beads under optimized binding conditions. Successive wash steps remove proteins, salts, cellular debris, and other contaminants, and the purified DNA is then eluted for immediate use in downstream applications.
Designed for both manual and automated processing, the kit provides a flexible and scalable approach for laboratories processing individual samples through high-throughput formats. For detailed automation protocols or platform-specific instructions, please contact AMD Biotech Technical Support at support@amdbiotech.com.
Features
- Broad Sample Compatibility
Efficiently isolates genomic DNA from whole blood, cultured cells, and a variety of animal tissues. - Efficient Contaminant Removal
Magnetic bead–based purification effectively removes proteins, salts, cellular debris, and other contaminants. - Downstream Application Ready
Purified DNA is suitable for a broad range of molecular biology applications - Flexible and Cost-Effective
Compatible with both manual and automated workflows, offering a cost-effective solution. - Alternative Workflow Option
Centrifugation-based protocol available when magnetic separation devices are not accessible
Product Contents
The kit contains the reagents required for sample lysis, genomic DNA capture, washing, and elution.
Manual Workflow for Blood DNA isolation
- Confirm the sample volume.
- Add Proteinase K to lysis the cells.
- Add Beads to bind DNA
- Separate beads from contaminants.
- Wash magnetic beads to remove contaminants.
- Elute DNA from magnetic beads
Data & References
Comparison of DNA Isolated from Whole Blood
Figure 1. Comparison of DNA Isolated from Whole Blood. gDNA was isolated from 50, 100 and 200 µL of whole blood using Auto-Mag® Blood & Tissue DNA Isolation Kit and a competitor’s M kit. Following isolation, 10 µL from each 100 µL elution was loaded on 1.2 % agarose gel, with the results indicating that the DNA isolation was consistent between the samples . In terms of determined recovery, Auto-Mag® Blood & Tissue DNA Isolation Kit shows a higher recovery than the competitor’s kit.
Specifications
- Isolation format:
- Magnetic bead-based technology
- Sample sources:
- Blood, saliva, tissue, cells, or buffy coat, etc
- Starting amount:
- Up to 200μl or 30mg
- Typical yield:
- Dependent upon sample, (~2-30μg)
- Purity:
- Typical Abs 260/280 ≥1.7
- Elution volume:
- >50μl
- Process method:
- Manual or automation
- Downstream application:
- PCR, Sequencing, Southern blotting, NGS, etc.
- Shipping/Shelf life:
- Shipping at ambient temperature/Stable for 12 months after the date of shipment.
- Over 450,000 preps sold!