Auto-Mag® Carboxyl Magnetic Beads (C-5)

Auto-Mag® Carboxyl Magnetic Beads (C-5) are  advanced  superparamagnetic iron oxide microspheres with a carboxyl-functionalized surface, optimized for efficient biomolecule binding and separation. These beads are highly versatile, capable of capturing and purifying a wide range of targets such as proteins, antigens, antibodies, nucleic acids (DNA/RNA), and cells. Their innovative design ensures faster binding kinetics with high sensitivity and selectivity, making them ideal for various biomedical and research applications. Compatible with both automated and manual workflows, Auto-Mag® Carboxyl Magnetic Beads (C-5) provide a reliable solution for achieving precise and efficient results.

Auto-Mag® Carboxyl Magnetic Beads (C-5) deliver superior yield, purity, and quality compared to leading competitors. Their unmatched value stems from in-house design and manufacturing, ensuring stringent quality control, enhanced performance, and cost efficiency.

Features

• Superparamagnetic Nature
  • Facilitates rapid magnetic separation.
  • Non-aggregating design ensures consistent performance.
• Carboxyl Functionalization
  • The surface is coated with free carboxyl groups (-COOH), which provide excellent reactivity for covalent coupling with biomolecules such as proteins, peptides, and nucleic acids.
• Proprietary Technology
  • Ensures stability and durability in various experimental conditions.
  • Proprietary coating technology prevents aggregation, ensuring a uniform bead suspension for
• Automation Compatibility
  • Suitable for automated extraction systems, enabling high-throughput workflows and large-scale operations.

Applications

• Genomic DNA and RNA Extraction
  • Compatible with DNA, RNA or plasmid DNA extraction protocols.
  • Ideal for isolating high-purity nucleic acids for downstream use.
• Molecular Biology Workflows:
  • Supports precision in sensitive assays like qPCR, RT-PCR, and sequencing.
• Biomolecule Conjugation:
  • Useful for immobilizing ligands, proteins, or other biomolecules for targeted experiments.

Benefits

• Fast and efficient separation process.
• No need for a centrifuge, simplifying workflows and reducing equipment dependency.
• Consistently high performance with low background interference
• Reliable results for demanding molecular biology applications.
• Enhanced performance and reliability make these beads competitive in the market

Workflow of Magnetic Beads RNA Extraction 

• Cell Lysis: Samples are lysed to release DNA/RNA.
• Add Beads: Add appropriate volume of beads into lysis.
• Binding: DNA/RNA binds to the Carboxyl magnetic beads in the binding buffer.
• Remove DNA & Washing: DNA was digested and then removed with ethanol-based wash buffers.
• Elution: Pure total RNA is eluted using a low-salt buffer or water.

Data & References

Total RNA Isolated from Blood & Tissue Samples with Auto-Mag® Carboxyl Magnetic Beads (C-5)

Figure 1. Total RNA are isolated from blood and tissue samples using the Total RNA Isolation Kit with Auto-Mag® Carboxyl Magnetic Beads (C-5), following the AMD Total RNA Isolation Kit standard protocol. Extractions were performed in triplicate, and each extracted RNA sample was analyzed individually using the Agilent TapeStation 2200. The figure illustrates the analysis of the extracted total RNA, which demonstrates high-quality rRNA with a RIN greater than 8.5.