Auto-Mag® Cell-free DNA (cfDNA) Isolation Kit
Product Information
Auto-Mag® Cell-free DNA (cfDNA) Isolation Kit is designed for efficient isolation and concentration of circulating cell-free DNA from plasma, serum, and urine. The optimized magnetic bead–based chemistry enables effective recovery of low-abundance cfDNA while minimizing carryover of contaminants and unwanted high-molecular-weight genomic DNA. The flexible workflow accommodates sample volumes from 0.2 to 10 mL, allowing laboratories to scale input according to sample availability and analytical requirements. Purified cfDNA can be recovered in a low elution volume, providing concentrated DNA suitable for sensitive downstream molecular analysis, including qPCR, DNA sequencing, microarrays, and NGS. etc.
Designed for both manual and automated workflows, the kit provides a practical and scalable solution for liquid biopsy research, biomarker analysis, and other applications requiring reliable recovery of cell-free nucleic acids.
Features
- Efficient cfDNA Recovery
Optimized magnetic bead chemistry enables reliable isolation of low-abundance cell-free DNA from plasma, serum, and urine. - Scalable Sample Input
Supports sample volumes from 0.2 to 10 mL, providing flexibility for different sample types and experimental requirements. - High Input, Low-Volume Elution
Process larger sample volumes and recover purified cfDNA in as little as 25–100 µL, helping concentrate low-abundance DNA for downstream analysis. - Reduced Genomic DNA Carryover
Optimized sample preparation and binding conditions help minimize contamination from high-molecular-weight genomic DNA. - Flexible Processing Options
Compatible with manual and automated fixed-volume workflows, with an optional centrifugation-based protocol available for specific processing requirements.
Product Contents
The Auto-Mag® Cell-free DNA (cfDNA) Isolation Kit contains the reagents required for sample lysis, cfDNA capture, washing, and elution.
Manual Workflow for cfDNA isolation
- Collect the sample.
- Prepare the cell-free supernatant.
- Add the binding buffer and magnetic beads to capture cfDNA.
- Separate the beads from impurities.
- Wash the magnetic beads to remove residual contaminants.
- Elute the purified cfDNA from the magnetic beads.
Specifications
- Isolation method:
- Magnetic bead-based technology
- Sample sources:
- Serum, Plasma or Urine
- Starting amount:
- 0.2 ml to 10 ml
- Downstream application:
- Next generation sequencing, Real time PCR and PCR
- Process method:
- Automated or manual
- Shipping:
- Ships at ambient temperature.
- Over 80,000 preps sold!