Auto-Mag® DNA Normalization Kit

Key Features (V.3.0) :

• Rapid & Reliable: Quantify and normalize DNA samples with confidence—whether genomic DNA, PCR products, NGS libraries, or fragmented DNA.
• One-Step Simplicity: Eliminate centrifugation and filtration. Purification, normalization, and recovery are completed in a single step, with no re-testing or re-quantification required.
• Proven Results: Delivers consistently reproducible outcomes, yielding ~400 ng of normalized DNA using the standard protocol.
• Flexible by Design: Recovery quantity can be customized to meet specific experimental needs.
• Seamless Integration: Compatible with both manual and automated workflows for effortless adoption.

Auto-Mag® DNA Normalization Kit utilizes proprietary magnetic bead technology to precisely limit DNA binding capacity, leaving excess DNA unbound. This enables rapid and consistent recovery of a predetermined amount of DNA from samples containing varying concentrations of genomic DNA (gDNA), PCR amplicons, NGS libraries, or other DNA fragments.

Unlike traditional DNA quantification methods, which require generating standard curves from known concentrations and extensive comparison with test samples—demanding significant effort and reagent use—the Auto-Mag® DNA Normalization Kit offers a simple, fast, centrifugation-free workflow. No additional DNA quantification or dilution steps are required, and the resulting normalized DNA is immediately ready for downstream applications such as PCR, sequencing, and NGS library preparation.

For gDNA normalization, the protocol recovers approximately 400 ng or 200 ng of normalized gDNA. For PCR amplicons and NGS library products, the protocol is designed to recover approximately 400 ng of normalized DNA. Custom protocols for other recovery amounts are available upon request. The kit (Cat. #S006-01P / -02P) also includes unbound DNA recovery beads, which allow for recovery of excess unbound DNA during the normalization process. The kit supports flexible processing formats, including 96-well plate (manual or automated) and single-tube (manual only) workflows.

Applications:

gDNA Normalization

Figure 1. Genomic DNA (gDNA) normalization was performed using 50 µL of gDNA from varying input amounts (samples 1–4) according to the standard protocol. Normalized gDNA was eluted in 40 µL of elution buffer, analyzed on a 1.2% agarose gel, and quantified with the Invitrogen Qubit assay to determine recovery yield. Samples 1-1 to 1-4 represent results from the 400 ng gDNA normalization protocol, while samples 2-1 to 2-4 represent results from the 200 ng protocol. These results demonstrate that the expected normalized DNA yield can be consistently achieved regardless of input DNA amount.

PCR Amplicon Normalization

Figure 2. The AMP region (~500 bp) of the pUC19 plasmid was amplified using AMP-specific primers in 50 µL PCR reactions. Aliquots of 50, 40, 35, and 25 µL of the original PCR products (samples 1–4) were processed and recovered using the standard PCR product normalization protocol. Following normalization, the recovered PCR products were eluted in 40 µL of elution buffer. Samples were analyzed by electrophoresis on a 1.2% agarose gel and quantified with the Invitrogen Qubit assay to determine recovery yield. The results (samples 4–8) demonstrate that the expected recovery of approximately 400 ng of normalized PCR product was consistently achieved across the tested input volumes (25–50 µL).