Auto-Mag® DNA Normalization Kit

The Auto-Mag® DNA Normalization Kit is a paramagnetic bead-based kit that can quickly and consistently recover a predetermined amount of DNA (output) from samples with varying amounts of genomic DNA, PCR amplicons, or DNA fragments (input).

• Rapid and reliable quantitation and normalization of gDNA, PCR amplicon, or fragmented DNA.
• No centrifugation step, no filtration step, and no standard curve.
• Multiple simultaneous actions: Normalization, clean-up, and concentration.
• Allowing the processing of pool DNA samples from various sources.
• Consistent normalization result
• Reduce library construction time, reagents usage and overall costs.
• Manual and automation-friendly.

Typically, DNA normalization occurs during the purification process. Standard methods for DNA quantification require the use of standard curves from DNA samples of known concentration.  Processing DNA samples from a variety of sources requires significant amounts of labor and reagent usage. The proprietary technology of the Auto-Mag® DNA Normalization Kit allows for the recovery of a predetermined amount of DNA, regardless of the amount of DNA in the sample, while minimizing labor and reagent requirements.

The principle of this DNA normalization method is based on the limitation of the DNA binding capacity of the proprietary beads. Excess unbound DNA can be separated and washed away, and a predetermined amount of DNA is recovered. Following the standard protocol, the normalized DNA yield is approximately 400ng per reaction (gDNA) and 400ng per reaction (PCR amplicons). If necessary, the normalized DNA yield can be adjusted to meet the needs of the end user. The normalization procedure is simple and centrifugation and filtration free. Additional DNA quantification and dilution is not necessary after DNA normalization, saving time and operating costs. In the process of normalization, the target DNA is not only normalized, but the DNA is also further purified and concentrated. The recovered DNA can be directly used in various subsequent reactions, such as PCR, sequencing, NGS library construction, or other applications, etc.

The Auto-Mag® DNA Normalization Kit can be performed in 96-well plates (manual and automatic) and single tubes (manual only) using the relevant protocol.

The kit also provides an Unbound DNA Recovery Reagent (available upon request) that can be used to recover excess DNA that was not bound during the DNA normalization process.

gDNA Normalization

Fig 1. gDNA normalization was performed using 20 µl of gDNA at different concentration input according to manufacturer’s recommended standard protocol. The normalized gDNA were eluted in 20 µl and analyzed on an 1.2% agarose gel electrophoreses.