Auto-Mag® DNA Normalization Kit

Auto-Mag® DNA Normalization Kit provides a simple and efficient magnetic bead–based method for normalizing DNA samples with different starting concentrations to a more consistent DNA output.

The optimized normalization chemistry uses controlled DNA-binding capacity to selectively capture a defined amount of DNA while leaving excess DNA unbound in samples containing sufficient input DNA. Following magnetic capture, washing, and elution, samples with variable starting DNA amounts are normalized toward a more uniform DNA output, minimizing the need for individual dilution or concentration adjustments.

For genomic DNA (gDNA), the standard protocols are designed to recover approximately 200 ng or 400 ng of normalized DNA. For PCR amplicons and NGS library products, the standard protocol is designed to recover approximately 400 ng of normalized DNA. Custom protocols targeting other DNA recovery amounts are available upon request.

The kit (Cat. #S006-01P / S006-02P) also includes unbound DNA recovery beads, allowing excess unbound DNA to be recovered during the normalization process when desired. Flexible processing formats include 96-well plate workflows for manual or automated processing and single-tube workflows for manual processing.

Auto-Mag® DNA Normalization Kit provides a practical and scalable solution for both manual and automated sample processing. The streamlined magnetic bead–based workflow is particularly well suited for high-throughput genotyping, sequencing, PCR, and other molecular biology applications requiring consistent DNA input across multiple samples.

Features

• Normalize Variable DNA Inputs
  Reduces differences among DNA samples with varying starting concentrations to provide a more consistent output for downstream analysis.
• Purification & Normalization in One Workflow
  Combines magnetic bead DNA cleanup with normalization, simplifying sample preparation.
• Controlled DNA Binding
  Optimized bead capacity limits DNA recovery above the normalization threshold, helping standardize DNA amounts across samples with sufficient input.
• Reduce Manual Dilution Steps
  Minimizes repetitive sample-by-sample dilution and concentration adjustment.
• Improve Workflow Consistency
  More uniform DNA inputs can simplify setup of downstream PCR, genotyping, sequencing, and other molecular workflows.
• High-Throughput Ready
  Particularly useful for multi-sample and 96-well workflows where individual DNA normalization can be labor-intensive.
• Automation-Ready
  Compatible with manual magnetic separation and automated magnetic bead–based liquid-handling systems.

gDNA Normalization

Figure 1. Genomic DNA (gDNA) normalization was performed using 50 µL of gDNA from varying input amounts (samples 1–4) according to the standard protocol. Normalized gDNA was eluted in 40 µL of elution buffer, analyzed on a 1.2% agarose gel, and quantified with the Invitrogen Qubit assay to determine recovery yield. Samples 1-1 to 1-4 represent results from the 400 ng gDNA normalization protocol, while samples 2-1 to 2-4 represent results from the 200 ng protocol. These results demonstrate that the expected normalized DNA yield can be consistently achieved regardless of input DNA amount.

PCR Amplicon Normalization

Figure 2. The AMP region (~500 bp) of the pUC19 plasmid was amplified using AMP-specific primers in 50 µL PCR reactions. Aliquots of 50, 40, 35, and 25 µL of the original PCR products (samples 1–4) were processed and recovered using the standard PCR product normalization protocol. Following normalization, the recovered PCR products were eluted in 40 µL of elution buffer. Samples were analyzed by electrophoresis on a 1.2% agarose gel and quantified with the Invitrogen Qubit assay to determine recovery yield. The results (samples 4–8) demonstrate that the expected recovery of approximately 400 ng of normalized PCR product was consistently achieved across the tested input volumes (25–50 µL).