Auto-Mag® Dried Blood Spot DNA Isolation Kit
Product Information
Auto-Mag® Dried Blood Spot DNA Isolation Kit is designed for efficient purification of genomic DNA from dried blood spot (DBS) samples. The optimized workflow combines effective DNA release from dried blood matrices with magnetic bead–based capture and purification, enabling reliable DNA recovery from limited starting material.
Following sample preparation and lysis, genomic DNA is captured onto magnetic beads under optimized binding conditions. Subsequent wash steps effectively remove proteins, salts, blood-derived contaminants, and other potential inhibitors. Purified DNA is then eluted and is suitable for a wide range of downstream molecular biology applications, including PCR, qPCR, genotyping, Southern blotting, microarray analysis, sequencing, and Next-Generation Sequencing (NGS).
Auto-Mag® Dried Blood Spot DNA Isolation Kit supports both manual and automated workflows, providing a simple, reliable, and scalable solution for laboratories processing freshly prepared or archived DBS specimens. For detailed operating instructions or protocols tailored to specific automation platforms, please contact AMD Biotech Technical Support at support@amdbiotech.com.
Features
- Optimized for Dried Blood Spots
Purpose-designed sample preparation and lysis conditions enable efficient DNA release from dried blood spot (DBS) matrices. - Efficient DNA Recovery from Limited Samples
Optimized magnetic bead chemistry provides reliable capture and recovery of genomic DNA from limited starting material. - Effective Contaminant Removal
Optimized purification efficiently removes proteins, salts, blood-derived contaminants, and other potential inhibitors that may interfere with downstream amplification and analysis. - Low-Volume DNA Recovery
Purified DNA can be recovered in a low elution volume, providing concentrated DNA for downstream applications where template availability is limited. - Flexible & Cost-Effective Processing
Supports both manual and automated workflows, providing a flexible and cost-effective solution for different sample throughput requirements. - Alternative Centrifugation-Based Method
An optional centrifugation-based protocol is available for laboratories without access to a magnetic separation device.
Product Contents
The Auto-Mag® Dried Blood Spot DNA Isolation Kit contains the reagents required for DBS sample lysis, genomic DNA capture, washing, and elution.
Manual Workflow for Dried Blood Spot DNA isolation
- Cut 3-6 pieces from a dried blood spot and place them in a Nuclease-free 1.5-2.0ml tube
- Add Proteinase K & lysis buffer to lysis the cells.
- Add Beads to bind DNA
- Separate beads from contaminants.
- Wash magnetic beads to remove contaminants.
- Elute DNA from magnetic beads.
Specifications
- Isolation method:
- Magnetic bead-based technology
- Sample sources:
- Dried blood samples
- Typical yield:
- Dependent upon sample, (~2-20μg)
- Process method:
- Manual or automation
- Downstream application:
- qPCR, PCR, Sequencing, NGS, etc.
- Shipping/Shelf life:
- Shipping at ambient temperature/Stable for 12 months after the date of shipment.