Auto-Mag® Dried Blood Spot DNA Isolation Kit
Product Information
Highlight
- Optimized Performance
Designed for efficient DNA extraction from dried blood spot punches - Fast & Easy Workflow
Simple and rapid processing with a magnetic bead–based system - High-Purity DNA
Effectively removes contaminants and inhibitors, with no RNA contamination - Broad Downstream Compatibility
Purified DNA is ready for PCR, qPCR, DNA ligation, sequencing, labeling, NGS, and more - Flexible Processing Options
Compatible with both manual and automated workflows in a cost-effective format - Alternative Method Available
Includes a centrifugation-based protocol when a magnetic separation device is unavailable
Auto-Mag® Dried Blood Spot DNA Isolation Kit is designed for efficient purification of high-quality genomic DNA from dried blood spot (DBS) samples. Using advanced lysis chemistry and Auto-Mag® magnetic bead technology, the kit delivers consistent and reliable DNA isolation. The purified DNA is suitable for a wide range of downstream applications, including real-time PCR, Southern blotting, microarray analysis, and Next-Generation Sequencing (NGS).
Auto-Mag® Dried Blood Spot DNA Isolation Kit supports both manual and automated workflows. It is fully compatible with platforms such as the KingFisher™ series, the IsoPure automated purification system, and most magnetic rod–based liquid handling systems. For detailed operating instructions or protocols tailored to specific platforms, please contact AMD Biotech Technical Support at support@amdbiotech.com.
For added flexibility, the kit also includes an alternative centrifugation-based protocol using 1.5 mL tubes when magnetic separation equipment is unavailable, without compromising performance.
Manual Workflow for Dried Blood Spot DNA isolation
- Cut 3-6 pieces from a dried blood spot and place them in a Nuclease-free 1.5-2.0ml tube
- Add Proteinase K & lysis buffer to lysis the cells.
- Add Beads to bind DNA
- Separate beads from contaminants.
- Wash magnetic beads to remove contaminants.
- Elute DNA from magnetic beads.
Specifications
- Isolation method:
- Magnetic bead-based technology
- Sample sources:
- Dried blood samples
- Typical yield:
- Dependent upon sample, (~2-20μg)
- Process method:
- Manual or automation
- Downstream application:
- qPCR, PCR, Sequencing, NGS, etc.
- Shipping/Shelf life:
- Shipping at ambient temperature/Stable for 12 months after the date of shipment.