Auto-Mag® FFPE Tissue DNA Isolation Kit
Product Information
Auto-Mag® FFPE Tissue DNA Isolation Kit is designed for efficient purification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples. The kit features an optimized workflow using a proprietary xylene-free deparaffinization reagent that is fully compatible with the extraction buffer. FFPE sample pretreatment, deparaffinization, tissue lysis, protein digestion, and magnetic bead–based DNA purification can be performed in a single tube, minimizing sample handling and sample loss while reducing the risk of cross-contamination.
The optimized purification chemistry provides high DNA recovery while effectively removing paraffin-related contaminants, proteins, salts, and other potential inhibitors. The purified DNA is suitable for a wide range of downstream molecular biology applications, including qPCR, mutation analysis, microarray analysis, sequencing, SNP genotyping, and STR genotyping. DNA yield, integrity, and downstream performance may vary depending on the fixation conditions, storage history, and quality of the original FFPE sample.
Formalin fixation can cause DNA–protein cross-linking and DNA fragmentation, while paraffin embedding introduces additional challenges during sample processing. Auto-Mag® FFPE Tissue DNA Extraction Kit provides an optimized solution for these challenging sample types and supports both manual and automated workflows. For detailed instructions or protocols tailored to specific automation platforms, please contact AMD Biotech Technical Support at support@amdbiotech.com.
Features
- Optimized for FFPE Samples
Purpose-designed sample preparation and purification conditions enable efficient DNA recovery from formalin-fixed, paraffin-embedded (FFPE) tissues. - Xylene-Free Workflow
Proprietary xylene-free deparaffinization eliminates the need for toxic organic solvents, providing safer and more convenient sample processing. - Single-Tube Processing
Deparaffinization, tissue lysis, protein digestion, and DNA purification are performed in a single tube, minimizing sample handling and sample loss while reducing the risk of cross-contamination. - Effective Contaminant Removal
Optimized purification efficiently removes proteins, salts, paraffin-associated contaminants, and other potential inhibitors that may interfere with downstream analysis. - Efficient DNA Recovery
Magnetic bead–based purification provides reliable DNA capture and recovery from limited and challenging FFPE specimens. - Manual or Automated Processing
Compatible with manual magnetic separation and readily adaptable to automated magnetic bead–based purification workflows.
Product Contents
The Auto-Mag® FFPE Tissue DNA Isolation Kit contains the reagents required for FFPE tissue processing, DNA release, magnetic bead capture, washing, and elution.
FFPE DNA isolation workflow with single tube
- Scrape FFPE tissue from FFPE slides or blokes.
- Deparaffinize with FFPE Deparaffinization Reagent (FDR)
- Lysis sample and decrosslink
- Add beads for DNA binding
- Separate beads from contaminants.
- Wash magnetic beads to remove contaminants.
- Elute DNA from magnetic beads.
Specifications
- Isolation method:
- Magnetic bead-based technology
- Sample sources:
- Formalin-fixed, paraffin-embedded tissue section samples.
- Starting amount:
- Dependent upon sample
- Safe Workflow:
- Xylene-free deparaffinization
- Downstream application:
- NGS, qPCR, mutation screening, microarray analysis, etc.
- Process method:
- Manual or automation
- Shipping/Shelf life:
- Shipping at ambient temperature/Stable for 12 months after the date of shipment.
- Over 15,000 preps sold!