Auto-Mag® FFPE Tissue DNA Isolation Kit
Product Information
Highlights
- Xylene-Free Workflow
Eliminates exposure to toxic chemicals for safer sample processing - Integrated Dewaxing System
No need to remove dewaxing reagents during extraction, simplifying the workflow and enabling faster turnaround - Single-Tube Processing
All steps are performed in a single centrifuge tube, minimizing sample loss and preventing cross-contamination - Consistent and Reliable Performance
Supports NGS, qPCR, and other downstream applications with reproducible results - Flexible and Automation-Ready
Compatible with both manual and automated platforms, delivering consistent DNA quality across workflows
Auto-Mag® FFPE Tissue DNA Isolation Kit utilizes paramagnetic bead–based technology to efficiently extract DNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples—without the use of xylene. The kit includes a proprietary dewaxing reagent that is fully compatible with the extraction buffers, eliminating the need for fluid transfers and enabling the entire workflow to be completed within a single centrifuge tube. This design minimizes sample loss and prevents cross-contamination between samples. The purified DNA offers high yield, excellent integrity, and is free of inhibitors, making it suitable for a wide range of downstream applications, including qPCR, mutation analysis, microarray analysis, sequencing, SNP genotyping, and STR genotyping.
Auto-Mag® FFPE Tissue DNA Isolation Kit supports both manual operation and automated workflows. It is fully compatible with platforms such as the KingFisher™ series, the IsoPure automated purification system, and most magnetic rod–based liquid handling systems. For detailed operating instructions or protocols tailored to specific platforms, please contact AMD Biotech Technical Support at support@amdbiotech.com
FFPE DNA isolation workflow with single tube
- Scrape FFPE tissue from FFPE slides or blokes.
- Deparaffinize with FFPE Deparaffinization Reagent (FDR)
- Lysis sample and decrosslink
- Add beads for DNA binding
- Separate beads from contaminants.
- Wash magnetic beads to remove contaminants.
- Elute DNA from magnetic beads.
Specifications
- Isolation method:
- Magnetic bead-based technology
- Sample sources:
- Formalin-fixed, paraffin-embedded tissue section samples.
- Starting amount:
- Dependent upon sample
- Safe Workflow:
- Xylene-free deparaffinization
- Downstream application:
- NGS, qPCR, mutation screening, microarray analysis, etc.
- Process method:
- Manual or automation
- Shipping/Shelf life:
- Ships at ambient temperature/12 months from date of delivery
- Over 15,000 preps sold!