Auto-Mag® FFPE Tissue DNA Isolation Kit

Auto-Mag® FFPE Tissue DNA Isolation Kit utilizes the paramagnetic bead-based technology and is specially designed to isolate DNA from formalin-fixed, paraffin-embedded (FFPE) tissue without the use of xylene. The Kit provides a special deparaffinization reagents and compatible extraction buffer for DNA isolation.  This combination allows the entire process of isolation to be performed in the same tube, minimizing sample loss and cross-contamination. The purified DNA is of high yield and integrity and is free of inhibitors, ready for use in several downstream applications including qPCR, mutation screening, microarray analysis, sequencing, single nucleotide polymorphism (SNP) and short-tandem repeat (STR) genotyping, etc.

• No Xylene – avoid toxic chemicals.
• No centrifuge needed and Automation-friendly.
• No need rid of deparaffinization reagent during isolation processing.
• Sample loss and cross contamination is eliminated as each sample remains in the same tube device during all isolation steps.
• Minimal steps required and convenient processing makes it suitable for all levels of expertise.
• Eluted DNA is suitable for most molecular downstream applications.

The isolation can be performed in both 96-well plates (manually and automated) and in 1.5-2.0 ml tubes (manually only) with related protocols.  Nucleic acid extraction begins with the solubilization of the paraffin from the tissue slices first. An enzymatic lysis step digests the tissue and releases the nucleic acids, followed by decrosslinking at a high temperature.  The binding solution is added to immobilize the nucleic acids to the surface of the Auto-Mag® D-3 beads. Contaminants are rinsed away using a simple washing procedure and the nucleic acids are eluted with elution buffer.

FFPE DNA isolation workflow with single tube 

1. Scrape FFPE tissue from FFPE slides or blokes.
2. Deparaffinize with FFPE Deparaffinization Reagent (FDR)
3. Lysis sample and decrosslink 
4. Add beads for DNA binding
5. Separate beads from contaminants.
6. Wash magnetic beads to remove contaminants.
7. Elute DNA from magnetic beads.