Auto-Mag® Agarose Gel DNA Extraction Kit

Highlights

• Rapid DNA Recovery
  Quick isolation of ultra-pure DNA fragments from agarose gels
• High Specificity Binding
  Efficient DNA binding enables effective removal of impurities and inhibitors
• Centrifugation-Free Workflow
  No centrifugation or filtration required in the standard protocol
• Downstream Application Ready
  Eluted DNA is suitable for ligation, sequencing, labeling, PCR, and other applications

Auto-Mag® Agarose Gel DNA Extraction Kit utilizes specially coated magnetic beads and an optimized buffer system for rapid purification of high-quality DNA from TAE- or TBE-buffered agarose gels. The workflow avoids the use of toxic reagents and enables efficient recovery of DNA fragments ranging from 100 bp to 20 kb, with yields exceeding 80%. The purified DNA is suitable for downstream applications such as ligation, PCR, sequencing, restriction digestion, and labeling reactions.

The kit includes a standard protocol for use with 1.5 mL tubes, as well as an alternative procedure that does not require magnetic separation devices, providing flexibility for different laboratory setups.

Gel DNA Extraction Workflow

1. Use a sterile scalpel or razor blade to excise the desired DNA band from the gel.
2. Add an appropriate volume of gel-dissolving buffer
3. Add beads to bind nucleic acids.
4. Separate the beads from contaminants
5. Wash magnetic to remove contaminants.
6. Elute DNA from magnetic beads.