Auto-Mag® Agarose Gel DNA Extraction Kit

• Quick recovery of ultra-pure DNA fragments from agarose gels
• Highly specific binding of DNA allows easy removal of impurities and inhibitors
• No need for centrifugation or filtration in standard extraction procedure.
• Eluted DNA is suitable for DNA ligation, sequencing, labeling, PCR, etc.

Auto-Mag® Agarose Gel DNA extraction Kit uses specially coated magnetic beads and the corresponding buffer system that provides rapid purification of high-quality DNA from TAE/TBE-buffered agarose gels. The process does not involve toxic reagents and is able to recover DNA fragments of 100 bp to 20 kb in size with yields exceeding 80%. The eluted DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions

The kit also provides the procedure for DNA Extraction by using 1.5 ml tubes and alternative procedure for DNA Extraction without using magnetic separation devices.

Gel DNA Extraction Workflow

1. Use a sterile scalpel or razor blade to excise the desired DNA band from the gel.
2. Add an appropriate volume of gel-dissolving buffer
3. Add beads to bind nucleic acids.
4. Separate the beads from contaminants
5. Wash magnetic to remove contaminants.
6. Elute DNA from magnetic beads.