Auto-Mag® Agarose Gel DNA Extraction Kit
Product Information
Auto-Mag® Agarose Gel DNA Extraction Kit provides a simple and efficient method for recovering DNA fragments from TAE- or TBE-buffered agarose gels. The optimized workflow combines rapid gel dissolution with magnetic bead–based DNA capture, enabling efficient recovery of excised DNA while removing agarose, salts, staining-related components, and other contaminants. Following excision of the desired DNA band, the agarose gel slice is dissolved using the supplied solubilization buffer. DNA is then captured onto magnetic beads, washed to remove residual contaminants, and eluted in a low-salt buffer or nuclease-free water for immediate downstream use.
The kit includes a standard protocol for use with 1.5 mL tubes, as well as an alternative procedure that does not require magnetic separation devices, providing flexibility for different laboratory setups.
Features
- Rapid Gel Dissolution
Optimized solubilization conditions efficiently dissolve excised agarose gel slices and prepare DNA for magnetic bead capture - Efficient DNA Recovery
Optimized magnetic bead chemistry enables reliable capture and recovery of DNA fragments from dissolved agarose gel samples. - Effective Contaminant Removal
Removes agarose, salts, staining-related components, and other contaminants that may interfere with downstream enzymatic reactions. - Manual or Automated Processing
Magnetic bead purification provides flexibility for both routine manual processing and adaptable automated workflowsl - Downstream Application Ready
Eluted DNA is suitable for ligation, sequencing, labeling, PCR, and other applications
Product Contents
The Auto-Mag® Agarose Gel DNA Extraction Kit contains the reagents required for agarose gel dissolution, DNA capture, washing, and elution.
Gel DNA Extraction Workflow
- Use a sterile scalpel or razor blade to excise the desired DNA band from the gel.
- Add an appropriate volume of gel-dissolving buffer
- Add beads to bind nucleic acids.
- Separate the beads from contaminants
- Wash magnetic to remove contaminants.
- Elute DNA from magnetic beads.
Specifications
- Purification method:
- Magnetic bead-based technology
- Sample sources:
- Agarose Gel
- Starting amount:
- Up to 500mg
- Purity:
- Typical Abs 260/280 ≥1.7
- Elution volume:
- >50μl
- Process method:
- Manual or automation
- Downstream application:
- Ligation, PCR, sequencing, restriction digestion, qPCR, PCR
- Shipping/Shelf life:
- Shipping at ambient temperature/Stable for 12 months after the date of shipment.