Auto-Mag® Micro Size Select

• Specially designed magnetic bead-based reagent for small size DNA, ssDNA, Oligo, or RNA cleanup and size selection 
  
  • dsDNA fragments 15bp or longer
  • ssDNA fragments 20nt or longer
  • Oligo and chimeric oligo 20nt or longer
  • RNA fragments 20nt or longer
• Fragment size selection of short DNA and RNA samples 
  • DNA Fragment Size select ranger: 15bp up
  • RNA fragment 25nt -200nt
• Expectant recover yield: Up to 95%
• Compatible with manual and automated processing and cost effective.

Auto-Mag® Micro Size Select reagent is specifically designed to recover and purify small DNA fragments as small as 15 bp from sheared DNA, PCR or RT-PCR reactions, and enzyme digestion products. A key feature of this reagent is its ability to selectively recover DNA fragments of a specific size or size range (15 bp–300 bp) by adjusting the volume ratio of Auto-Mag® Micro Size Select to the DNA sample, similar to double-sided size selection protocols. The purified small DNA fragments are suitable for various applications, including PCR, fragment analysis, fluorescent or radioactive sequencing, capillary electrophoresis, DNA labeling, and ligation.

Additionally, Auto-Mag® Micro Size Select is an RNase-free reagent, making it ideal for miRNA enrichment and RNA size selection from total RNA, broadening its applicability in RNA-based workflows.

Using advanced magnetic bead technology, Auto-Mag® Micro Size Select is compatible with both manual procedures and automated liquid-handling workstations, offering versatility and efficiency for diverse laboratory needs.

Notice of New Product Packaging Size

To provide customers with greater flexibility and additional packaging options, a new 50 mL package size has been added to this product line. The catalog number for the new 50 mL package is S004-02. Accordingly, the catalog number for the original 100 mL package has been updated to S004-03. This change applies only to the packaging size and catalog number. There are no changes to the product formulation, specifications, performance, quality, or instructions for use.

Small size DNA Fragments or RNA cleanup & size selection Workflow

1. Confirm the DND/RNA sample volume.
2. Add appropriate volume of Auto-Mag® Micro Size Select to remove larger DNA/RNA fragments.
3. Separate the beads and transfer supernatant to a new vessels.
4. Add new Auto-Mag® Micro Size Select to the supernatant to recover desired size DNA/RNA.
5. Separate the beads from contaminants.
6. Wash magnetic beads with 85% ethanol to remove contaminants.
7. Elute size-selective DNA or RNA from magnetic beads.

Data & Refrences

Small size DNA fragment size selection

Fig 1. Auto-Mag® Micro Size Select performs double-sided size selection on sheared DNA to recover small DNA fragments. A series of different volume ratios of Auto-Mag® Micro Size Select reagent to sample were used to selectively recover small DNA fragments of different size ranges. The recovered DNA was analyzed on the Agilent TapeStation® 2200.

Recovery effects of different ratio for Small DNA fragment size selection

Fig-2-1. Auto-Mag® Micro Size Select performs double-sided size selection (0.4x/1.6x) to recover <300bp fragments from DNA ladder mix. The recovered DNA was analyzed on the Agilent TapeStation® 2200. The DNA recovery rate is over 90% on the desired target range.

Fig-2-2. Auto-Mag® Micro Size Select performs double-sided size selection (0.6x/1.4x) to recover <200bp fragments from DNA ladder mix. The recovered DNA was analyzed on the Agilent TapeStation® 2200. The DNA recovery rate is over 90% on the desired target range.

Fig-2-3. Auto-Mag® Micro Size Select performs double-sided size selection (0.8x/1.2x) to recover <100bp fragments from DNA ladder mix. The recovered DNA was analyzed on the Agilent TapeStation® 2200. The DNA recovery rate is over 90% on the desired target range.

Fig-2-4. Auto-Mag® Micro Size Select performs double-sided size selection (1.0x/1.0x) to recover <75bp fragments from DNA ladder mix. The recovered DNA was analyzed on the Agilent TapeStation® 2200. The DNA recovery rate is over 90% on the desired target range.

Fig-2-5. Auto-Mag® Micro Size Select performs double-sided size selection (1.2x/0.8x) to recover <50bp fragments from DNA ladder mix. The recovered DNA was analyzed on the Agilent TapeStation® 2200. The DNA recovery rate is over 90% on the desired target range.