Auto-Mag® Micro Size Select (Micro-Select)

Auto-Mag® Micro RNA/DNA Size Select is designed to recover and purify small DNA fragments as small as 15bp from sheared DNA fragments, PCR or RT-PCR reactions, and enzyme digestion. Another notable feature is the selection of small-sized DNA fragments. Like the DNA double-sided size selection steps, the small DNA fragments of a specific size or size range (15bp-300bp) can be recovered from the sample by adjusting the volume ratio of Auto-Mag® Micro Size Select to the DNA sample. The purified small DNA fragments can be used for PCR, fragment analysis, fluorescent or radioactive sequencing, capillary electrophoresis, DNA labeling and ligation, etc.

Auto-Mag® Micro Size Select is an RNase free reagent that can be used for miRNA enrichment and RNA size selection from total RNA.

Auto-Mag® Micro Size Select uses magnetic technology, and it is suitable for manual procedures as well as for adapting it to automatic liquid handling workstations.

Features

• Effective cleanup and recover of short DNA and RNA samples
  • dsDNA fragments 15bp or longer
  • ssDNA fragments 20nt or longer
  • Oligo and chimeric oligo 20nt or longer
  • RNA fragments 20nt or longer
• Fragment size selection of short DNA and RNA samples 
  • DNA Fragment Size select ranger: 15bp up
  • RNA fragment 25nt -200nt
• Expectant recover Yield: Up to 95%
• Compatible with manual and automated processing and cost effective.

Product name change notice: To intuitively clarify the characteristics and functions of this product, the name of Auto-Mag® Micro Select  is changed to Auto-Mag® Micro Size Select. There are no changes in the characteristics, quality and usage of the product.

Small size DNA Fragments or RNA cleanup & size selection Workflow

1. Confirm the DND/RNA sample volume.
2. Add appropriate volume of Auto-Mag® Micro Size Select to remove larger DNA/RNA fragments.
3. Separate the beads and transfer supernatant to a new vessels.
4. Add new Auto-Mag® Micro Size Select to the supernatant to recover desired size DNA/RNA.
5. Separate the beads from contaminants.
6. Wash magnetic beads with 85% ethanol to remove contaminants.
7. Elute size-selective DNA or RNA from magnetic beads.

Data & Refrences

Small size DNA fragment size selection

Fig 1. Auto-Mag® Micro Size Select performs double-sided size selection on sheared DNA to recover small DNA fragments. A series of different volume ratios of Auto-Mag® Micro Size Select reagent to sample were used to selectively recover small DNA fragments of different size ranges. The recovered DNA was analyzed on the Agilent TapeStation® 2200.

Recovery effects of different ratio for Small DNA fragment size selection

Fig-2-1. Auto-Mag® Micro Size Select performs double-sided size selection (0.4x/1.6x) to recover <300bp fragments from DNA ladder mix. The recovered DNA was analyzed on the Agilent TapeStation® 2200. The DNA recovery rate is over 90% on the desired target range.

Fig-2-2. Auto-Mag® Micro Size Select performs double-sided size selection (0.6x/1.4x) to recover <200bp fragments from DNA ladder mix. The recovered DNA was analyzed on the Agilent TapeStation® 2200. The DNA recovery rate is over 90% on the desired target range.

Fig-2-3. Auto-Mag® Micro Size Select performs double-sided size selection (0.8x/1.2x) to recover <100bp fragments from DNA ladder mix. The recovered DNA was analyzed on the Agilent TapeStation® 2200. The DNA recovery rate is over 90% on the desired target range.

Fig-2-4. Auto-Mag® Micro Size Select performs double-sided size selection (1.0x/1.0x) to recover <75bp fragments from DNA ladder mix. The recovered DNA was analyzed on the Agilent TapeStation® 2200. The DNA recovery rate is over 90% on the desired target range.

Fig-2-5. Auto-Mag® Micro Size Select performs double-sided size selection (1.2x/0.8x) to recover <50bp fragments from DNA ladder mix. The recovered DNA was analyzed on the Agilent TapeStation® 2200. The DNA recovery rate is over 90% on the desired target range.