Auto-Mag® Micro Size Select (Micro-Select)

• Specially designed magnetic bead-based reagent for small size DNA, ssDNA, Oligo, or RNA cleanup and size selection 
  
  • dsDNA fragments 15bp or longer
  • ssDNA fragments 20nt or longer
  • Oligo and chimeric oligo 20nt or longer
  • RNA fragments 20nt or longer
• Fragment size selection of short DNA and RNA samples 
  • DNA Fragment Size select ranger: 15bp up
  • RNA fragment 25nt -200nt
• Expectant recover yield: Up to 95%
• Compatible with manual and automated processing and cost effective.

Auto-Mag® Micro Size Select reagent is specifically designed to recover and purify small DNA fragments as small as 15 bp from sheared DNA, PCR or RT-PCR reactions, and enzyme digestion products. A key feature of this reagent is its ability to selectively recover DNA fragments of a specific size or size range (15 bp–300 bp) by adjusting the volume ratio of Auto-Mag® Micro Size Select to the DNA sample, similar to double-sided size selection protocols. The purified small DNA fragments are suitable for various applications, including PCR, fragment analysis, fluorescent or radioactive sequencing, capillary electrophoresis, DNA labeling, and ligation.

Additionally, Auto-Mag® Micro Size Select is an RNase-free reagent, making it ideal for miRNA enrichment and RNA size selection from total RNA, broadening its applicability in RNA-based workflows.

Using advanced magnetic bead technology, Auto-Mag® Micro Size Select is compatible with both manual procedures and automated liquid-handling workstations, offering versatility and efficiency for diverse laboratory needs.

Product name change notice: To intuitively clarify the characteristics and functions of this product, the name of Auto-Mag® Micro Select  is changed to Auto-Mag® Micro Size Select. There are no changes in the characteristics, quality and usage of the product.

Small size DNA Fragments or RNA cleanup & size selection Workflow

1. Confirm the DND/RNA sample volume.
2. Add appropriate volume of Auto-Mag® Micro Size Select to remove larger DNA/RNA fragments.
3. Separate the beads and transfer supernatant to a new vessels.
4. Add new Auto-Mag® Micro Size Select to the supernatant to recover desired size DNA/RNA.
5. Separate the beads from contaminants.
6. Wash magnetic beads with 85% ethanol to remove contaminants.
7. Elute size-selective DNA or RNA from magnetic beads.

Data & Refrences

Small size DNA fragment size selection

Fig 1. Auto-Mag® Micro Size Select performs double-sided size selection on sheared DNA to recover small DNA fragments. A series of different volume ratios of Auto-Mag® Micro Size Select reagent to sample were used to selectively recover small DNA fragments of different size ranges. The recovered DNA was analyzed on the Agilent TapeStation® 2200.

Recovery effects of different ratio for Small DNA fragment size selection

Fig-2-1. Auto-Mag® Micro Size Select performs double-sided size selection (0.4x/1.6x) to recover <300bp fragments from DNA ladder mix. The recovered DNA was analyzed on the Agilent TapeStation® 2200. The DNA recovery rate is over 90% on the desired target range.

Fig-2-2. Auto-Mag® Micro Size Select performs double-sided size selection (0.6x/1.4x) to recover <200bp fragments from DNA ladder mix. The recovered DNA was analyzed on the Agilent TapeStation® 2200. The DNA recovery rate is over 90% on the desired target range.

Fig-2-3. Auto-Mag® Micro Size Select performs double-sided size selection (0.8x/1.2x) to recover <100bp fragments from DNA ladder mix. The recovered DNA was analyzed on the Agilent TapeStation® 2200. The DNA recovery rate is over 90% on the desired target range.

Fig-2-4. Auto-Mag® Micro Size Select performs double-sided size selection (1.0x/1.0x) to recover <75bp fragments from DNA ladder mix. The recovered DNA was analyzed on the Agilent TapeStation® 2200. The DNA recovery rate is over 90% on the desired target range.

Fig-2-5. Auto-Mag® Micro Size Select performs double-sided size selection (1.2x/0.8x) to recover <50bp fragments from DNA ladder mix. The recovered DNA was analyzed on the Agilent TapeStation® 2200. The DNA recovery rate is over 90% on the desired target range.