Auto-Mag® PCR-Pure

Auto-Mag® PCR-Pure Reagent utilizes advanced paramagnetic bead technology to enable efficient purification of PCR amplicons and precise size selection of DNA fragments, making it ideal for next-generation sequencing (NGS) library preparation.

With Auto-Mag® PCR-Pure, DNA fragments selectively bind to magnetic beads, while primers, dNTPs, salts, and other contaminants are effectively removed through simple wash steps. The purified DNA is then eluted in a low-salt buffer or water, ready for immediate use in downstream applications such as NGS library preparation, Sanger sequencing, cloning, restriction digestion, adapter ligation, and microarray analysis.

Designed for versatility, Auto-Mag® PCR-Pure is compatible with both manual workflows and automated systems, delivering a streamlined, reliable, and cost-effective solution for DNA purification.

Key Features

• Rapid and Reliable Post-PCR and DNA Cleanup
• No Protocol Changes Required: Fully compatible with established workflows from major competitors
• High Recovery Efficiency: Optimized for amplicons and dsDNA fragments >100 bp
• Efficient Contaminant Removal: Eliminates dNTPs, primers, primer dimers, salts, and other impurities
• Consistent Size Selection: Delivers predictable fragment distribution and reproducible results
• Automation-Ready: Easily adaptable to high-throughput liquid handling systems
• Cost-Effective Solution: Provides significant cost savings without compromising performance

PCR amplicon/DNA fragment purification Workflow

1. Bind DNA to magnetic beads
2. Separate beads from contaminants
3. Wash magnetic beads with 70% ethanol to remove contaminants
4. Elute DNA from magnetic beads
5. Transfer to new storage vessels

Data & References

PCR amplicons purified using  Auto-Mag® PCR-Pure with 1.8x ratio.

Fig 1. Demonstrate that the PCR amplicons purified using  Auto-Mag® PCR-Pure with 1.8x ratio. The purified PCR products were analyzed on the 1.2% agarose gel electrophoresis which shows that Auto-Mag® PCR-Pure reagent provides predictable performance while maintaining efficient recovery.

Comparison of purification and size selection 

Fig 2. Comparison of purification and size selection on 20μl of DNA ladder mix at 1.8x ratio set using  Auto-Mag® PCR-Pure reagent and  AMPure XP reagents following manufacturer’s recommended protocols. The purified DNA was eluted in 20μl and analyzed on Agilent's Tapestation® 2200. The results show that both  reagents provide predictable performance while maintaining efficient and similar recovery.