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Auto-Mag® PCR-Pure

Magnetic beads-based reagent for PCR clean up and DNA fragment size selection for NGS
Cat#: S002-01 Auto-Mag® PCR-Pure (5ml)
$58.00 USD
Quantity
Auto-Mag® PCR-Pure

Product Information

  • Rapid and reliable post-PCR and DNA clean-up reagent
  • No protocol change against major competitors
  • High recovery of amplicons or dsDNA fragments greater than 100bp
  • Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants
  • Predictable and consistent DNA size selection and uniform fragments size distribution
  • Adaptable to high throughput liquid handling workstations
  • Significant cost savings

The Auto-Mag® PCR-Pure Reagent leverages advanced paramagnetic bead technology for efficient purification of amplicons and precise size-selection of DNA fragments during library preparation for next-generation sequencing (NGS).

With Auto-Mag® PCR-Pure, DNA fragments selectively bind to the magnetic beads, while primers, dNTPs, and salts are effectively removed through simple wash steps. The resulting highly purified DNA is eluted in a low-salt buffer or water, ready for immediate use in downstream applications such as NGS library construction, Sanger sequencing, cloning, restriction digestion, adapter ligation, and microarray analysis.

Compatible with both manual workflows and automated systems, Auto-Mag® PCR-Pure provides a streamlined, reliable, and cost-effective solution for all your DNA purification needs.

PCR amplicon/DNA fragment purification Workflow

  1. Bind DNA to magnetic beads
  2. Separate beads from contaminants
  3. Wash magnetic beads with 70% ethanol to remove contaminants
  4. Elute DNA from magnetic beads
  5. Transfer to new storage vessels

Data & References

PCR amplicons purified using  Auto-Mag® PCR-Pure with 1.8x ratio.

Fig 1. Demonstrate that the PCR amplicons purified using  Auto-Mag® PCR-Pure with 1.8x ratio. The purified PCR products were analyzed on the 1.2% agarose gel electrophoresis which shows that Auto-Mag® PCR-Pure reagent provides predictable performance while maintaining efficient recovery.

 

Comparison of purification and size selection 

Fig 2. Comparison of purification and size selection on 20μl of DNA ladder mix at 1.8x ratio set using  Auto-Mag® PCR-Pure reagent and  AMPure XP reagents following manufacturer’s recommended protocols. The purified DNA was eluted in 20μl and analyzed on Agilent's Tapestation® 2200. The results show that both  reagents provide predictable performance while maintaining efficient and similar recovery.

 

Specifications

  • Purification method: Magnetic bead-based technology
  • Sample sources: gDNA, PCR products, cDNA, DNA fragments
  • Starting amount: Scalable
  • Recovery: Normal recovery rangers between 80-95% for PCR amplicons (>100bp)
  • Applications: PCR Purification, DNA Cleanup, NGS Cleanup
  • Operation: Ready-to-use at room temperature.
  • Process method: Manual or automation
  • Storage: 2-8°C. Ships at ambient temperature.
  • Shelf life: 12 months from date of delivery & 24 months from date of production.

Protocol / Handbooks / Resources

Frequently Asked Questions
Can Auto-Mag® PCR-Pure be used for DNA fragment size selection of NGS library preparation?
This product could perform purification and may have some size selection capabilities, but for more exact size selection range, Auto-Mag® X-Pure Size Select NGS (Cat# S003) reagent is specifically designed for DNA size selection, and is fully validated for this application.

Can the Auto-Mag® PCR-Pure be used to cleanup RNA?
Even though Auto-Mag® PCR-Pure could bind both DNA and RNA; however, we recommend using Auto-Mag® RNA-Pure (Cat# S008) reagent for RNA cleanup, which is manufactured under RNase-free conditions and is QC tested to be RNase free.

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