Auto-Mag® PCR-Pure
Overview
Auto-Mag® PCR-Pure reagent is based on paramagnetic bead technology designed for efficient purification of amplicons and size-selection of DNA fragments in library preparation for NGS.
- Works with DNA, DNA fragments
- No protocol change against major competitor
- High recovery of amplicons or dsDNA fragments greater than 100bp
- Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants
- Predictable and consistent DNA size selection and uniform fragments size distribution
- Adaptable to high throughput liquid handling workstations
- Significant cost savings
The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapter ligation, microarrays, and so on. Auto-Mag® PCR-Pure is suitable for manual procedures as well as in automation.
Manual Workflow for PCR amplicon/DNA fragment purification
- Bind DNA to magnetic beads
- Separate beads from contaminants
- Wash magnetic beads with 70% ethanol to remove contaminants
- Elute DNA from magnetic beads
- Transfer to new storage vessels
Data & References
PCR amplicons purified using Auto-Mag® PCR-Pure with 1.8x ratio.
Fig 1. Demonstrate that the PCR amplicons purified using Auto-Mag® PCR-Pure with 1.8x ratio. The purified PCR products were analyzed on the 1.2% agarose gel electrophoresis which shows that Auto-Mag® PCR-Pure reagent provides predictable performance while maintaining efficient recovery.
Comparison of purification and size selection
Fig 2. Comparison of purification and size selection on 20μl of DNA ladder mix at 1.8x ratio set using Auto-Mag® PCR-Pure reagent and AMPure XP reagents following manufacturer’s recommended protocols. The purified DNA was eluted in 20μl and analyzed on Agilent's Tapestation® 2200. The results show that both reagents provide predictable performance while maintaining efficient and similar recovery.
Specifications
- Technology: Paramagnetic bead-based technology
- Applications: PCR Purification, DNA Cleanup, NGS Cleanup
- Operation: Ready-to-use at room temperature.
- Starting Amount: Scalable
- Recovery: Normal recovery rangers between 80-95% for PCR amplicons (>100bp)
- Processing mode: Automated or manual
- Hands-on Time Manual Workflow (96 well ) ~30 minutes
- Storage: 2-8°C. Ships at ambient temperature.
- Shelf life: 24 months from date of production.