Auto-Mag® Plant Total RNA Isolation Kit
Product Information
- Purified PCR grade RNA with no inhibiting substances (polysaccharides and phenols)
- Robust lysis system (chemical lysis combined with a mechanical homogenization).
- Eluted RNA is well suitable for a wide range of downstream applications
- Compatible with manual and automated processing and cost-effective.
Auto-Mag® Plant Total RNA Isolation Kit is designed for the rapid and efficient purification of high-quality total RNA from a wide range of plant species and tissue types. Utilizing magnetic bead-based technology, the standard protocol allows for RNA isolation in less than 30 minutes following cell lysis. The kit supports processing in 96-well plates (manually or with automated systems) and 1.5 mL tubes (manual workflows only), with related protocols provided for flexibility. The purified RNA is of the highest integrity and is suitable for various downstream applications, including real-time PCR, Southern blotting, SNP analysis, next-generation sequencing (NGS), and hybridization-based studies.
The Auto-Mag® Plant Total RNA Isolation Kit is also compatible with high-throughput liquid handling workstations, making it ideal for laboratories requiring scalability and efficiency.
Plant Total RNA Extraction Workflow
- Disrupt plant sample.
- Add beads to bind nucleic acids.
- Separate the beads from contaminants
- Remove DNA
- Rebinding RNA
- Wash magnetic to remove contaminants.
- Elute RNA from magnetic beads.
Specifications
- Isolation method: Magnetic bead-based technology
- Sample sources: Plant tissue, seeds, etc.
- Starting amount: Up to 50mg
- Typical yield: Depend upon sample
- Elution volume: >50 µl
- RNA purity:OD260/280:1.9-2.0, OD260/230:1.7-2.0
- Process method: Manual or automation
- Downstream application: RT-PCR, Northern blotting. RNA-Seq. etc.
- Shelf life: 12 months from date of delivery & 24 months from date of production.