Auto-Mag® Plant Total RNA Isolation Kit
Product Information
Auto-Mag® Plant Total RNA Isolation Kit is designed for the rapid and efficient purification of high-quality total RNA from a wide variety of plant species and tissue types. Utilizing advanced magnetic bead–based technology, the standard workflow enables RNA isolation in less than 30 minutes after cell lysis. The purified RNA is of high integrity and suitable for a broad range of downstream applications, including real-time PCR, Southern blotting, SNP analysis, Next-Generation Sequencing (NGS), and hybridization-based studies.
The kit supports both manual and automated workflows and is fully compatible with automation platforms such as KingFisher™, the IsoPure Automated Purification System, and most magnetic rod–based liquid handling systems. For detailed operating instructions or platform-specific automation protocols, please contact the AMD Biotech support team at support@amdbiotech.com.
Features
- Broad Plant Tissue Compatibility
Efficiently isolates total RNA from leaves, stems, seeds, and a wide range of other plant tissues. - Efficient RNA Recovery
Optimized lysis conditions and magnetic bead chemistry enable reliable capture and recovery of high-quality total RNA from diverse plant samples. - Effective Contaminant Removal
Efficiently reduces proteins, salts, polysaccharides, polyphenols, and other plant-derived contaminants that may interfere with downstream applications. - On-Bead DNase I Treatment
Integrated on-bead DNase I treatment removes residual genomic DNA during magnetic bead purification, simplifying the workflow and minimizing sample handling. - Manual or Automated Processing
Compatible with conventional magnetic separation and automated magnetic bead-based purification platforms for flexible laboratory workflows. - High-Throughput & Cost-Effective
Easily adaptable to 96-well processing for high-throughput plant genomics and gene expression studies, providing a practical and cost-effective solution for routine RNA isolation.
Product Contents
The Auto-Mag® Plant Total RNA Isolation Kit contains the reagents required for plant sample lysis, RNA capture, DNase treatment, washing, and elution.
Plant Total RNA Extraction Workflow
- Disrupt plant sample.
- Add beads to bind nucleic acids.
- Separate the beads from contaminants
- Remove DNA
- Rebinding RNA
- Wash magnetic to remove contaminants.
- Elute RNA from magnetic beads.
Specifications
- Isolation method:
- Magnetic bead-based technology
- Sample sources:
- Plant tissue, seeds, etc.
- Starting amount:
- Up to 50mg
- Typical yield:
- Depend upon sample
- RNA purity:
- OD260/280:1.9-2.0, OD260/230:1.7-2.0
- Process method:
- Manual or automation
- Downstream application:
- RT-PCR, Northern blotting. RNA-Seq. etc.
- Shipping/Shelf life:
- Shipping at ambient temperature/Stable for 12 months after the date of shipment.