Auto-Mag® Plant Total RNA Isolation Kit
Overview
Auto-Mag® Plant Total RNA Isolation Kit is specially designed for rapid and efficient purification of high-quality total RNA from a wide variety of plant species and tissue types. The kit utilizes Magnetic bead-based technology, and the standard procedure takes less than 30 minutes following cell lysis. The isolation can be performed in both 96-well plates (manually and automated) and in 1.5 ml tubes (manually only) with related protocols. Plant samples are disrupted in a homogenizer/bead-based milling equipment first. PRL Buffer is added to lyse the samples. The supernatant is then transferred to a new processing plate where Auto-Mag® C-5 beads are added to bind to the RNA. Following DNA are digested and a few wash steps, the highest integrity purified RNA is eluted and can be used in a wide range of downstream applications, such as Real-time PCR, Southern blotting, SNP analysis, NGS, hybridization applications, and other RNA-based analysis. The Auto-Mag® Plant RNA Isolation Kit can be adapted to high throughput liquid handling workstations.
- Fast and easy processing using a magnetic bead system
- Robust lysis system – chemical lysis combined with a mechanical homogenization
- Consistent, high yields of total RNA
- Isolate high-quality total RNA from a variety of plant species.
- Eluted RNA is well suitable for wide range of downstream applications
Specifications
- Isolation Technology: Magnetic bead-based technology
- Sample Sources: Plant tissue, seeds, etc.
- Starting Amount: Up to 50mg
- Typical Yield: Depend upon sample
- Elution Volume: 50-100µl
- Processing format: Automated; Manual
- Downstream Application: RT-PCR, Northern blotting. RNA-Seq. etc.
- Shelf life: 24 months from date of production.