Auto-Mag® Plasmid DNA Miniprep Kit

Auto-Mag® Plasmid DNA Miniprep Kit utilized magnetic beads with alkaline-SDS lysis of bacterial cells to isolate high-quality plasmid DNA in a high-throughput format.

• Fast, reproducible, and easy processing using a magnetic bead system
• High quality plasmid DNA purification
• Recovered plasmid DNA is compatible with various downstream applications
• Adaptable to various automated liquid handling workstations
• No toxic organic solvents

By using a 96-well format, up to 96 samples can be simultaneously processed in less than 60 minutes. By utilizing magnetic beads chemistry, the system is geared for automation as the protocol requires no vacuum filtration. When used with high copy plasmids, Auto-Mag® Plasmid DNA Miniprep Kit yields ~5-15μg plasmid. Yields vary slightly depending on the cell line, vector type, and size of the construct. Purified plasmid DNA can be used in downstream applications directly.

The kit also provides the procedure for isolating plasmids DNA by using 1.5 ml tubes and alternative procedure for isolating plasmids DNA without using magnetic separation devices.

Plasmid DNA Miniprep Workflow

1. Prepare bacteria cultures & pellet the cells.
2. Add lysis buffers to lysis cells.
3. Separate cell debris
4. Add beads to supernatant to bind plasmid DNA.
5. Separate the beads from contaminants
6. Wash magnetic beads with 70% ethanol to remove contaminants.
7. Elute plasmid DNA from magnetic beads.