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Auto-Mag® Plasmid DNA Miniprep Kit

Magnetic beads based kit designed to extract high-quality plasmid DNA (including Cosmids, Fosmids, and BACs) from bacterial cultures.
Cat#: D014-01 Auto-Mag® Plasmid DNA Miniprep Kit (96Preps)
$75.00 USD $96.00 USD Save $21.00 USD
Quantity
Auto-Mag® Plasmid DNA Miniprep Kit

Product Information

  • High quality plasmid DNA purification using a magnetic bead system
  • High yields of both high and low copy number plasmids
  • Recovered plasmid DNA is suitable for most molecular downstream applications.
  • Compatible with manual and automated processing and cost-effective

The Auto-Mag® Plasmid DNA Miniprep Kit utilizes magnetic bead-based technology combined with alkaline-SDS lysis to efficiently isolate high-quality plasmid DNA in a high-throughput format.

With its 96-well format, the kit can process up to 96 samples simultaneously in under 60 minutes. The magnetic bead chemistry eliminates the need for vacuum filtration, making the system ideal for automation. For high-copy plasmids, the kit yields approximately 5–15 µg of plasmid DNA per sample, with yields varying depending on the cell line, vector type, and construct size. The purified plasmid DNA is ready for direct use in downstream applications.

The kit also includes protocols for isolating plasmid DNA in 1.5 mL tubes, as well as an alternative procedure that does not require magnetic separation devices, offering flexibility for various laboratory setups.

Plasmid DNA Miniprep Workflow

  1. Prepare bacteria cultures & pellet the cells.
  2. Add lysis buffers to lysis cells.
  3. Separate cell debris
  4. Add beads to supernatant to bind plasmid DNA.
  5. Separate the beads from contaminants
  6. Wash magnetic beads with 70% ethanol to remove contaminants.
  7. Elute plasmid DNA from magnetic beads.

Data & References

Concentration Comparison

Figure 1. pUC-19 plasmid was purified from 1 mL DH5α cultures harboring the plasmid and eluted in 100 µL volume using kits from Auto-Mag® Plasmid DNA Miniprep Kit and a competitor kit according to manufacturer’s recommended protocols.

Figure Left: Plasmid DNA concentration was determined by optical density measurements using Thermo Scientific’s NanoDrop™ 2000c system. In terms of determined recovery, Auto-Mag® Plasmid DNA Miniprep Kit shows a higher recovery than the competitor’s kit.

Figure Right: Purified Plasmids are cut with BamH1 and, 10 µL from each sample was analyzed on a 1.2% Agarose gel.

 

Plasmid sequncing data

Figure 2.  Plasmid DNA purified with Auto-Mag® Plasmid DNA Miniprep Kit was used in a  Sanger sequencing reaction.  DNA was analyzed on an Applied Biosystems 3730XL. Chromatograms show continuous reads close to 1,000 base in length with uniform signal intensity and very low noise or background.

 

Specifications

  • Isolation method: Magnetic bead-based technology
  • Main function: Isolation of low/high copy plasmid DNA
  • Sample sources: Bacterial cultures
  • Starting amount: 0.5 to 1.5 mL of culture with OD600 between 2 and 3
  • Typical yield: Dependent upon sample, (~2-15μg)
  • Elution volume: 50-100μl
  • Purity: A260/280: 1.7-1.9
  • Process method: Manual or automation
  • Downstream application: PCR, Cloning, Sequencing, Transformation, Labeling, NGS.
  • Shelf life: 12 months from date of delivery & 24 months from date of production.
  • Over 55,000 preps sold!

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