Auto-Mag® Plasmid DNA Miniprep Kit

Auto-Mag® Plasmid DNA Miniprep Kit provides a rapid and reliable solution for purification of high-quality plasmid DNA from bacterial cultures. The optimized workflow combines alkaline lysis with magnetic bead–based DNA purification, enabling efficient plasmid recovery while removing proteins, salts, genomic DNA, and other cellular contaminants.
The kit typically provides 3–20 µg of purified plasmid DNA, depending on plasmid copy number, bacterial strain, culture conditions, and sample input. Purified DNA is suitable for a broad range of downstream molecular biology applications.
Designed for both manual and automated processing, Auto-Mag® Plasmid DNA Miniprep Kit eliminates conventional spin-column purification and provides a scalable solution for processing individual samples through high-throughput multiwell formats.

Features 

• High-Quality Plasmid DNA Purification
  Magnetic bead–based system for reliable and efficient Plasmid DNA isolation
• High Yield Performance
  Up to 20 µg of plasmid DNA from 1.5 mL TB culture. 
• Transfection-Quality DNA
  Endotoxin-free plasmid DNA with endotoxin levels <0.1 EU/µg
• High Purity
  Contaminant-free DNA with A260/A280 >1.7 and A260/A230 >1.8
• Downstream Application Ready
  Suitable for a wide range of molecular biology applications
• Flexible and Cost-Effective
  Compatible with manual and automated processing and cost-effective

Product Contents

Auto-Mag® Plasmid DNA Miniprep Kit contains the reagents required for bacterial cell lysis, plasmid DNA capture, washing, and elution.

Plasmid DNA Miniprep Workflow

1. Prepare bacteria cultures & pellet the cells.
2. Add lysis buffers to lysis cells.
3. Separate cell debris
4. Add beads to supernatant to bind plasmid DNA.
5. Separate the beads from contaminants
6. Wash magnetic beads with 70% ethanol to remove contaminants.
7. Elute plasmid DNA from magnetic beads.

Data & References

Concentration Comparison

Figure 1. pUC-19 plasmid was purified from 1 mL DH5α cultures harboring the plasmid and eluted in 100 µL volume using kits from Auto-Mag® Plasmid DNA Miniprep Kit and a competitor kit according to manufacturer’s recommended protocols.

Figure Left: Plasmid DNA concentration was determined by optical density measurements using Thermo Scientific’s NanoDrop™ 2000c system. In terms of determined recovery, Auto-Mag® Plasmid DNA Miniprep Kit shows a higher recovery than the competitor’s kit.

Figure Right: Purified Plasmids are cut with BamH1 and, 10 µL from each sample was analyzed on a 1.2% Agarose gel.

Plasmid sequncing data

Figure 2.  Plasmid DNA purified with Auto-Mag® Plasmid DNA Miniprep Kit was used in a  Sanger sequencing reaction.  DNA was analyzed on an Applied Biosystems 3730XL. Chromatograms show continuous reads close to 1,000 base in length with uniform signal intensity and very low noise or background.