Auto-Mag® Plasmid DNA Miniprep Kit

Highlights

• High-Quality Plasmid DNA Purification
  Magnetic bead–based system for reliable and efficient DNA isolation
• High Yield Performance
  Up to 20 µg of plasmid DNA from 1.5 mL TB culture. 
• Transfection-Quality DNA
  Endotoxin-free plasmid DNA with endotoxin levels <0.1 EU/µg
• High Purity
  Contaminant-free DNA with A260/A280 >1.7 and A260/A230 >1.8
• Downstream Application Ready
  Suitable for a wide range of molecular biology applications
• Flexible and Cost-Effective
  Compatible with manual and automated processing and cost-effective

Auto-Mag® Plasmid DNA Miniprep Kit (Version 3.0) integrates nanomagnetic bead technology, alkaline SDS lysis, and an endotoxin-free plasmid binding system to isolate high-quality, low-endotoxin plasmid DNA from up to 1.5 mL of overnight E. coli culture. The purified plasmid DNA is ready for immediate use in downstream applications. The kit is compatible with both magnetic bead–based and centrifugation-based workflows, and is easily adaptable to high-throughput automated platforms, providing flexibility across diverse laboratory environments.

For detailed automation protocols or platform-specific instructions, please contact AMD Biotech Technical Support at support@amdbiotech.com

Plasmid DNA Miniprep Workflow

1. Prepare bacteria cultures & pellet the cells.
2. Add lysis buffers to lysis cells.
3. Separate cell debris
4. Add beads to supernatant to bind plasmid DNA.
5. Separate the beads from contaminants
6. Wash magnetic beads with 70% ethanol to remove contaminants.
7. Elute plasmid DNA from magnetic beads.

Data & References

Concentration Comparison

Figure 1. pUC-19 plasmid was purified from 1 mL DH5α cultures harboring the plasmid and eluted in 100 µL volume using kits from Auto-Mag® Plasmid DNA Miniprep Kit and a competitor kit according to manufacturer’s recommended protocols.

Figure Left: Plasmid DNA concentration was determined by optical density measurements using Thermo Scientific’s NanoDrop™ 2000c system. In terms of determined recovery, Auto-Mag® Plasmid DNA Miniprep Kit shows a higher recovery than the competitor’s kit.

Figure Right: Purified Plasmids are cut with BamH1 and, 10 µL from each sample was analyzed on a 1.2% Agarose gel.

Plasmid sequncing data

Figure 2.  Plasmid DNA purified with Auto-Mag® Plasmid DNA Miniprep Kit was used in a  Sanger sequencing reaction.  DNA was analyzed on an Applied Biosystems 3730XL. Chromatograms show continuous reads close to 1,000 base in length with uniform signal intensity and very low noise or background.