Auto-Mag® Plasmid DNA Midiprep Kit

• Fast and Reproducible: Streamline plasmid DNA purification with a magnetic bead-based system designed for quick and consistent results.
• High-Quality DNA Output: Achieve superior purity levels, ensuring reliable performance in downstream applications.
• Broad Application Compatibility: Purified DNA is ready for use in molecular workflows such as sequencing, PCR, cloning, and transfection.
• Flexible Workflow Options: Suitable for both manual and automated processes, offering cost-effective solutions for diverse laboratory needs.

Auto-Mag® Plasmid DNA Midi Prep Kit is a versatile magnetic bead-based system for efficient, large-scale purification of high-quality plasmid DNA from recombinant bacterial cultures. It can isolate 80–250 μg of high-copy or 10–50 μg of low-copy plasmid DNA from 10–50 mL of overnight culture, ensuring excellent yield and purity. The resulting plasmid DNA is immediately ready for use in a range of downstream applications, including fluorescent DNA sequencing, restriction enzyme digestion, PCR, cloning, transformation, mammalian cell transfection, and more. For added convenience, the kit also offers a non-magnetic separation protocol, enhancing flexibility for different lab workflows and equipment setups.

Plasmid DNA Midi-Prep Workflow

1. Prepare bacteria cultures & pellet the cells.
2. Add lysis buffers to lysis cells.
3. Separate cell debris
4. Add beads to supernatant to bind plasmid DNA.
5. Separate the beads from contaminants
6. Wash magnetic beads with 70% ethanol to remove contaminants.
7. Elute plasmid DNA from magnetic beads.