Auto-Mag® Plasmid DNA Midiprep Kit

Auto-Mag® Plasmid DNA Midi Prep Kit is magnetic beads-based plasmid purification system and designed for large scale isolation of high-quality plasmid DNA from recombinant bacteria cultures.

• Fast, reproducible, and easy processing using a magnetic bead system
• High quality plasmid DNA purification
• Recovered plasmid DNA is compatible with various downstream applications
• Adaptable to various automated liquid handling workstations
• No toxic organic solvents

Up to 80-250μg high copy number plasmid DNA or 10-50μg low copy number plasmid DNA can be purified from 10-50 ml overnight culture. Purified plasmid DNA can be used in a wide variety of downstream applications directly, such as fluorescent DNA sequencing, restriction endonuclease digestion, PCR, cloning, transformation, transfection of mammalian cells, and other manipulations.

The kit also provides the procedure for isolating plasmids DNA without using magnetic separation devices.

Plasmid DNA Midi-prep Workflow

1. Prepare bacteria cultures & pellet the cells.
2. Add lysis buffers to lysis cells.
3. Separate cell debris
4. Add beads to supernatant to bind plasmid DNA.
5. Separate the beads from contaminants
6. Wash magnetic beads with 70% ethanol to remove contaminants.
7. Elute plasmid DNA from magnetic beads.