Auto-Mag® RNA-Pure

Auto-Mag® RNA-Pure provide a fast and simple paramagnetic bead-based reagent for RNA cleanup and concentration after any enzymatic reaction (including in vitro transcription, DNase I treatment, capping and labeling) and after other purification methods such as phenol/chloroform extraction. The kit also can use to selectively recover different sizes range RNA from total RNA samples in NGS based procedures by using supplementary protocol.

• Works with RNA and cDNA
• No centrifugation step, no filtration step
• Compatible with manual and automated processing
• Rapid and reliable clean-up total RNA, including miRNA, siRNA, and aRNA
• Complete removal of salts, unincorporated primers, and dNTPs
• Efficient purification of both large and small RNAs, and RNA size selection
• Used in RNA-Seq library preparations.

Auto-Mag® RNA-Pure can be used for manual procedures as well as for adapting to automated liquid handling instruments for high-throughput sample cleanup. The highly purified RNA can be used directly for downstream applications, such as PCR and RT-PCR reactions, RNase protection assays, Transfection for RNAi experiments, Antisense RNA (aRNA) amplification, Probes for microarray or microarray, cDNA synthesis and labeling, as well as RNA-Seq library preparations, etc.

Auto-Mag® RNA-Pure Manual Workflow

1.  Confirm the sample volume.
2.  Add appropriate volume of Auto-Mag® RNA-Pure to the sample.
3.  Bind RNA to magnetic beads
4.  Separate the beads from contaminants
5.  Wash magnetic beads with 85% ethanol to remove contaminants.
6.  Elute Purified RNA from magnetic beads.

Data & References 

Total RNA Cleanup & Purification

Fig 1:  The 20 µl of RNA at 20 ng/µl, 10µl, and 5 ng/µl was cleaned up with Auto-Mag® RNA-Pure reagent following recommended protocols. The RNA was eluted in 20 µl and analyzed on Agilent’s TapeStation® 2200. Recovery rates ranged over 85% respectively.