Auto-Mag® RNA-Pure

• Designed direct, cost-efficient alternative to the Benchmark
• Rapid and reliable RNA and cDNA clean-up & concentration reagent
• Complete removal of salts, unincorporated primers and dNTPs
• Used in RNA-Seq library preparations, Next-Gen Sequencing, RT-qPCR, hybridization, etc.
• Compatible with manual and automated processing and cost-effective

Auto-Mag® RNA-Pure offers a fast and simple paramagnetic bead-based solution for RNA cleanup and concentration after enzymatic reactions (such as in vitro transcription, DNase I treatment, capping, and labeling) or following other purification methods like phenol/chloroform extraction. The kit can also be used to selectively recover RNA of different size ranges from total RNA samples in NGS-based procedures, using an additional supplementary protocol.

Auto-Mag® RNA-Pure is compatible with both manual workflows and automated liquid-handling instruments for high-throughput sample cleanup. The highly purified RNA can be directly utilized in a variety of downstream applications, including PCR and RT-PCR reactions, RNase protection assays, RNAi transfection experiments, antisense RNA (aRNA) amplification, microarray probes, cDNA synthesis and labeling, as well as RNA-Seq library preparation, among others.

Total RNA Cleanup Workflow

1.  Confirm the sample volume.
2.  Add appropriate volume of Auto-Mag® RNA-Pure to the sample.
3.  Bind RNA to magnetic beads
4.  Separate the beads from contaminants
5.  Wash magnetic beads with 85% ethanol to remove contaminants.
6.  Elute Purified RNA from magnetic beads.

Data & References 

Total RNA Cleanup & Purification

Fig 1:  The 20 µl of RNA at 20 ng/µl, 10ng/µl, and 5ng/µl was cleaned up with Auto-Mag® RNA-Pure reagent following recommended protocols. The RNA was eluted in 20 µl and analyzed on Agilent’s TapeStation® 2200. Recovery rates ranged over 85% respectively.