Auto-Mag® Soil/Fecal DNA Isolation Kit

• Fast and easy processing using a magnetic bead system
• Contains pre-filled 2 ml disruptor tube
• Reproducible DNA Isolation from a variety of sample sources
• High yields of inhibitor-free, PCR-quality DNA and ready for downstream applications

Auto-Mag® Soil/Fecal DNA Isolation Kits are specifically designed for the efficient and rapid isolation of inhibitor-free, PCR-quality DNA from microbial populations present in soil and fecal samples.

Utilizing proprietary IHR Reagents (Inhibitor and Humic Acid Removal Reagent), the kit effectively removes humic substances and other PCR inhibitors. It is compatible with a diverse range of soil types—including clay, silty, sandy, peaty, chalky, and loamy—as well as fecal material from various sources, including humans, rodents, birds, and livestock. The high-quality DNA obtained is suitable for a wide array of downstream molecular applications, such as PCR, microarray analysis, genotyping, methylation profiling, Sanger sequencing, and next-generation sequencing (NGS).

The Auto-Mag® platform utilizes nanomagnetic bead technology and features an optimized workflow that supports both manual and automated workflow.

Soil/Fecal DNA Isolation Workflow

1. Disrupt the samples.
2. Add lysis buffer and Proteinase K to lyse the samples.
3. Add IHR reagent to remove potential inhibitors.
4. Centrifuge and transfer the supernatant.
5. Add binding buffer and magnetic beads to capture nucleic acids.
6. Separate the magnetic beads from contaminants.
7. Wash the magnetic beads to remove residual impurities.
8. Elute the DNA from the magnetic beads.

Data & References