Auto-Mag® Soil/Fecal DNA Isolation Kit

Highlights

• Fast and Easy Workflow
  Magnetic bead–based system enables rapid and straightforward processing
• Pre-Filled Disruptor Tubes
  Includes 2 mL disruptor tubes for efficient sample homogenization
• Reproducible DNA Isolation
  Delivers consistent performance across a wide range of soil and fecal sample types
• High-Quality DNA Output
  Provides high yields of inhibitor-free, PCR-quality DNA ready for downstream applications

Auto-Mag® Soil/Fecal DNA Isolation Kit is designed for the rapid and efficient isolation of inhibitor-free, PCR-quality DNA from microbial populations in soil and fecal samples. Utilizing proprietary IHR Reagents (Inhibitor and Humic Acid Removal), the kit effectively eliminates humic substances and other PCR inhibitors. It is compatible with a wide range of soil types—including clay, silty, sandy, peaty, chalky, and loamy soils—as well as fecal samples from humans, rodents, birds, and livestock. The purified DNA is suitable for a broad range of downstream applications, including PCR, microarray analysis, genotyping, methylation profiling, Sanger sequencing, and next-generation sequencing (NGS).

Auto-Mag® Soil/Fecal DNA Isolation Kit is compatible with both manual and automated workflows, providing a reliable, scalable, and cost-effective solution for modern molecular biology applications.

For detailed automation protocols or platform-specific instructions—including for the KingFisher™ system—please contact AMD Biotech Technical Support at support@amdbiotech.com

Soil/Fecal DNA Isolation Workflow

1. Disrupt the samples.
2. Add lysis buffer and Proteinase K to lyse the samples.
3. Add IHR reagent to remove potential inhibitors.
4. Centrifuge and transfer the supernatant.
5. Add binding buffer and magnetic beads to capture nucleic acids.
6. Separate the magnetic beads from contaminants.
7. Wash the magnetic beads to remove residual impurities.
8. Elute the DNA from the magnetic beads.

Data & References