Auto-Mag® Total RNA Isolation Kit

Auto-Mag® Total RNA Isolation Kit provides a simple and reliable magnetic bead-based solution for the purification of total RNA from cultured cells, animal tissues, and other compatible biological samples. The kit combines an optimized lysis process with on-bead DNase I treatment to efficiently remove residual genomic DNA and isolate high-quality total RNA. The purified RNA is suitable for a wide range of downstream applications, including RNA-Seq, RT-PCR, RT-qPCR, NGS, gene expression analysis, and hybridization-based assays.

Auto-Mag® Total RNA Isolation Kit is fully compatible with both manual and automated workflows, providing a flexible and scalable solution for routine and high-throughput RNA purification. For detailed operating instructions and protocols for specific automation platforms, please contact AMD Biotech Technical Support at support@amdbiotech.com.

Features

• Broad Sample Compatibility
  Designed for efficient total RNA isolation from cultured cells, animal tissues, and other compatible biological samples.
• Efficient Total RNA Recovery
  Optimized magnetic bead chemistry enables reliable capture and recovery of high-quality total RNA from a variety of biological samples.
• Effective Contaminant Removal
  Efficiently removes proteins, salts, and other contaminants that may interfere with sensitive downstream molecular applications.
• On-Bead DNase I Treatment
  Integrated on-bead DNase I treatment efficiently removes residual genomic DNA during purification while simplifying the overall workflow.
• Manual or Automated Processing
  Compatible with manual magnetic separation and automated magnetic bead-based purification platforms for flexible laboratory workflows.
• High-Throughput & Cost-Effective
  Easily adaptable to 96-well processing for high-throughput gene expression and sequencing workflows, providing a practical and cost-effective solution for routine total RNA isolation.

Product Contents

Auto-Mag® Total RNA Isolation Kit contains the reagents required for sample lysis, RNA capture, washing, and elution.

Total RNA Extraction Workflow

1. Disrupt and Lysis tissue or cells.
2. Add beads to bind nucleic acids.
3. Separate the beads from contaminants
4. Remove DNA
5. Rebinding RNA
6. Wash magnetic to remove contaminants
7. Elute RNA from magnetic beads

Data & References

Total RNA Isolated from Blood & Tissue Samples

Figure 1. Total RNAs are isolated from blood and tissue samples using the Auto-Mag® Total RNA Isolation Kit) following the AMD’s Total RNA Isolation Kit standard protocol. Extractions were performed in triplicate, and each extracted RNA sample was analyzed individually using the Agilent TapeStation 2200. The figure illustrates the analysis of the extracted total RNA, which demonstrates high-quality RNA with a RIN greater than 8.5.