Auto-Mag® Quantitative Normalized Plasmid DNA Prep Kit

Highlights

• All-in-One Workflow
  Magnetic bead–based process that integrates plasmid extraction and DNA normalization into a single streamlined workflow
• Consistent, Quantitative Yield
  Standard protocol delivers ~2 µg of endotoxin-free (<0.1 EU/µg) plasmid DNA per sample
• Scalable and Reproducible
  Recovery yields can be scaled to meet specific requirements while maintaining consistent performance across large sample batches
• Flexible Operation and Automation-Ready
  Compatible with both magnetic bead–based and centrifugation-based workflows, and easily adaptable to high-throughput automated platforms

Auto-Mag® Quantitative Normalized Plasmid DNA Prep Kit integrates classic alkaline SDS lysis, an advanced endotoxin removal wash system, and AMD’s proprietary superparamagnetic bead–based DNA normalization technology to enable rapid, streamlined, and quantitative isolation of high-quality, endotoxin-free (<0.1 EU/µg) plasmid DNA from bacterial cultures.

Engineered for simplicity and efficiency, the standard workflow consistently yields approximately 2 µg of plasmid DNA (2–10 kb) per preparation with minimal sample-to-sample variation and performs reliably even with larger input volumes. The streamlined protocol reduces hands-on time and minimizes the risk of user error, making it ideal for high-throughput laboratories and routine molecular biology applications.

The purified, normalized plasmid DNA is immediately ready for downstream applications, including cloning, sequencing, transfection, and research in biomedicine, gene expression, antibiotic resistance, and gene therapy development.

Optional protocols are available to accommodate a wide range of DNA recovery requirements, including plasmids larger than 10 kb. The kit supports both magnetic bead–based and centrifugation-based workflows, ensuring flexibility across manual and automated platforms. For additional information, please contact AMD Biotech.

Overview of the Workflow for Normalized Plasmid DNA Isolation

• Prepare bacterial cultures and pellet the cells by centrifugation.
• Lyse the cells using alkaline lysis buffer.
• Remove cellular debris and genomic DNA by centrifugation.
• Bind plasmid DNA with magnetic beads and normalization buffer.
• Separate beads magnetically and wash.
• Elute the normalized plasmid DNA.

Data & References

Normalized Plasmid DNA Isolation

Figure 1. Plasmid extraction was performed using an E. coli strain carrying the pUC19 plasmid cultured at 37°C for 16 hours to an OD₆₀₀ of 2.9. Different culture volumes (0.5–2 mL) were processed either with the standard plasmid extraction protocol (samples 1, 3, 5, and 7) or with the 2 µg quantitative normalized plasmid DNA preparation protocol (samples 2, 4, 6, and 8). Plasmid DNA was eluted in 100 µL of Elution buffer, analyzed by 1.2% agarose gel electrophoresis, quantified using the Invitrogen Qubit assay and Agilent TapeStation 2200, and assessed for residual endotoxin using a Chromogenic LAL Endotoxin Assay Kit.

The results show that control samples yielded variable amounts of plasmid DNA, ranging from 6.8 µg to 2.8 µg depending on the culture volume. In contrast, samples processed using the normalized plasmid DNA preparation protocol consistently produced approximately 2 µg of plasmid DNA across the tested culture volume range (0.5–2 mL). These results demonstrate that the Quantitatively Normalized Plasmid DNA Prep Kit reliably delivers high-quality plasmid DNA with consistent yield, independent of the starting culture volume.