Auto-Mag® Yeast DNA Miniprep Kit ( with 2 ml Prefilled Disruptor Tubes)

Highlights

• Efficient Yeast DNA Extraction
  Optimized for genomic DNA isolation from a wide range of fungal species
• Robust Lysis Performance
  Combines bead mill homogenization with enzymatic digestion for effective cell disruption
• High-Quality DNA Output
  Recovers up to 10 µg of genomic DNA from 1 × 10⁸ yeast cells. Yields DNA fragments typically ranging from 35–60 kb, suitable for diverse downstream applications
• Magnetic Bead-Based Workflow and Efficient Contaminant Removal
  Streamlined binding, washing, and elution steps ensure high-purity DNA
• Flexible, Scalable, and Automation-Ready
  Compatible with KingFisher™, IsoPure systems, and most magnetic rod–based liquid handlers, delivering consistent DNA quality in both manual and automated workflows

Auto-Mag® Yeast DNA Miniprep Kit utilizes advanced magnetic bead technology for the reliable and efficient isolation of genomic DNA from a wide range of fungal species. The workflow begins with lysis buffer addition, followed by bead mill homogenization and enzymatic digestion to ensure effective DNA release. Magnetic beads then capture the DNA under optimized binding conditions. After two rapid wash steps to remove salts and protein contaminants, the purified DNA is eluted and recovered. The extracted DNA typically ranges from 35 to 60 kb in size and is suitable for a variety of downstream applications, including PCR, restriction enzyme digestion, and hybridization assays.

Auto-Mag® Yeast DNA Miniprep Kit is fully compatible with automated workflows, including the KingFisher™ platform, the IsoPure automated purification system, and most magnetic rod–based liquid handling systems. For detailed protocols tailored to specific automation platforms, please contact AMD Biotech technical support at support@amdbiotech.com

 Yeast DNA Isolation Workflow

• Prepare yeast cultures & pellet the cells.
• Add disrupt lysis buffers and homogenize cells.
• Add beads to bind nucleic acids.
• Separate the beads from contaminants
• Wash magnetic to remove contaminants.
• Elute  DNA from magnetic beads.

Frequently Asked Questions (FAQ)

Q1. What strains is this kit compatible with?
This kit is compatible with a broad range of fungal and yeast species, including Ashbya, Kloeckera, Candida, Kluyveromyces, Debaryomyces, Lipomyces, Eremothecium, Metschnikowia, Endomyces, Pichia, Hansenula, Aureobasidium (Pullularia), Hanseniaspora, Saccharomyces, Saccharomycodes, Saccharomycopsis, Schizosaccharomyces, and Torulopsis.

Q2. What type of lysis method is used?
The workflow combines mechanical disruption (bead mill homogenization) with enzymatic digestion to ensure efficient lysis of yeast and fungal cells.

Q3. Does the kit support automation?
Yes, the kit is compatible with automated platforms such as KingFisher™, IsoPure systems, and most magnetic rod–based liquid handling instruments.