Auto-Mag® Yeast DNA Miniprep Kit ( with 2 ml Prefilled Disruptor Tubes)
Product Information
- Rapid and convenient method for isolating genomic DNA from yeast and fungal cell samples
- Recovers up to 10 µg of genomic DNA from 1 × 10⁸ yeast cells
- Produces high-quality DNA ready for PCR and other downstream applications
- Delivers equivalent DNA quality in both manual and automated extractions
- Suitable for magnetic-rod liquid handlers, including KingFisher™ and IsoPure systems
Auto-Mag® Yeast DNA Miniprep Kit utilizes advanced magnetic bead technology for the rapid and reliable isolation of high-quality total cellular DNA from various yeast species. The kit efficiently processes up to 1.5 mL of log-phase yeast culture (OD600 of 1.0 in YPD medium), making it ideal for streamlined and reproducible DNA extraction workflows.
Auto-Mag® Yeast DNA Miniprep Kit workflow begins by adding lysis buffer to the yeast sample and transferring the mixture to a disruptor tube. The sample is then mixed and heated for 5 minutes. Following this, the sample is homogenized for 5 minutes using an appropriate disruptor to ensure efficient cell lysis. Next, digestion enzymes are added to the sample, which is incubated at 55°C for 20 minutes to release the DNA effectively. The sample is then centrifuged, and the supernatant is transferred to a DNase-free microcentrifuge tube. For RNA-free genomic DNA, an optional RNase treatment can be performed at this stage. DNA is captured using magnetic beads in the presence of a binding buffer. After two quick wash steps to remove salts and protein contaminants, the purified DNA is eluted with an elution buffer. The resulting high-quality DNA is suitable for downstream applications such as PCR, restriction digestion, and hybridization techniques.
Auto-Mag® Yeast DNA Miniprep Kit is fully compatible with automated workflows, including KingFisher™ platforms, the IsoPure Automated Purification System, and most magnetic rod liquid handling systems. For detailed operating instructions and protocols for specific automation platforms, please contact the AMD Biotech support team at support@amdbiotech.com.
Yeast DNA Isolation Workflow
- Prepare yeast cultures & pellet the cells.
- Add disrupt lysis buffers and homogenize cells.
- Add beads to bind nucleic acids.
- Separate the beads from contaminants
- Wash magnetic to remove contaminants.
- Elute DNA from magnetic beads.
Specifications
- Isolation method: Magnetic bead-based technology
- Sample sources:Yeast
- Sample volume:≤ 1.5 ml of Culture
- Typical yield: Dependent upon sample,
- Process method: Manual or automation
- Application:PCR, restriction digestion, and hybridization techniques. NGS.
- Shipping Ships at ambient temperature
- Shelf life: 12 months from date of delivery & 24 months from date of production.