Linear Polyacrylamide Solution (LPA) (5mg/ml)
Overview
Linear Polyacrylamide (LPA) is an inert coprecipitate used to aid recovery of nucleic acids during alcohol precipitations, essential for quantitative recovery of small amounts of nucleic acids in dilute solutions. Relative to other carriers, such as tRNA or glycogen, LPA offers several advantages for recovering DNA or studying DNA-protein interactions. Linear polyacrylamide eliminates the risk of trace contaminants being introduced to the precipitation process because it is chemically synthesized and rigorously tested for nuclease contamination. There is no contamination from biological materials. Linear polyacrylamide does not inhibit enzymatic reactions, but also does not interfere with spectrophotometric readings at 260nm and 280nm. These distinct advantages make linear acrylamide the best choice for precipitating DNA and RNA for amplification reactions.
Features
- Quantitative recovery of low concentrations (ng/ml) of nucleic acid
- Prevents pellet loss in nuclease protection assays.
- Precipitate DNA fragments > 15 base pairs.
Applications
- For DNA/RNA precipitation,
- Coprecipitate
- PCR amplification
- Ligation
- Other applications where high purity and recovery are essential.