Auto-Mag® Water DNA/RNA Kit

Auto-Mag® Water DNA/RNA Kit is a novel, patent-pending solution for water sample microbial collection and nucleic acid extraction. Unlike traditional environmental microbial analysis methods, the kit utilizes specially modified magnetic beads to efficiently capture and enrich bacteria, pathogens, viruses, free nucleic acids, and other microorganisms from water samples. This makes the kit ideal for a wide range of environmental analyses.

Traditional methods for capturing microorganisms in water typically involve labor-intensive filtration and overnight culturing, which can be time-consuming and inefficient. The Auto-Mag® Kit simplifies this process by providing all necessary reagents for both the enrichment of microorganisms and the reliable extraction of RNA and DNA. This can be performed manually or through automated systems, making the process more efficient.

Purified nucleic acids (DNA/RNA) extracted using the kit are suitable for sensitive downstream applications, including digital droplet PCR (ddPCR), reverse transcription quantitative PCR (RT-qPCR), next-generation sequencing (NGS), and other advanced genomic technologies. Additionally, microorganisms captured by the magnetic beads can be directly inoculated for further separation, identification, and biological studies, enhancing the practical utility of the kit for microbial analysis.

Overall, the Auto-Mag® Water DNA/RNA Kit significantly improves efficiency, reduces labor, and offers a high degree of versatility, making it a powerful tool for microbial analysis in aquatic ecosystems.

Key Features:

• Rapid Enrichment:
  The kit uses specially designed paramagnetic beads to quickly enrich microorganisms and viruses in liquid samples, eliminating the need for traditional filtration or centrifugation methods. This allows for faster, more efficient microbial capture.

• Directly Inoculate Culture:
  The microorganisms enriched with magnetic beads can be directly cultured, isolated, and biologically identified, enabling routine microbial analysis without the need for additional preparation steps.

• Efficient Nucleic Acid Extraction:
  After the enrichment process, the kit’s lysis step releases nucleic acids, which then bind reversibly to the paramagnetic beads. The following washing and drying steps remove impurities, resulting in highly purified DNA/RNA, ready for further analysis.

• Versatile Application:
  The purified DNA/RNA can be used in a wide range of sensitive downstream applications such as real-time PCR, reverse transcription PCR (RT-PCR), and next-generation sequencing (NGS). These applications provide precise microbial identification and deeper insights into water ecosystems.

• Flexible and Automation-Ready:
  The Auto-Mag® Water DNA/RNA Kit is suitable for both manual use and integration into automated high-throughput workflows. It is compatible with most liquid handling systems and automatic magnetic separators, allowing for flexible operation in various laboratory settings. Processing times may vary depending on the instrument configuration and magnetic separation system. For automation integration and available scripts for specific platforms, users can contact AMD Biotech.

Water DNA Isolation Workflow

1. Collect and confirm the water sample volume.
2. Add appropriate volume of capture beads and capture enhancer to the sample.
3. Rotate the sample at room temperature for microorganisms' enrichment and capture
4. Separate and collect microorganism/beads complex
5. Culture microorganism Directly 
6. Or go to nucleic acid isolation processing

Data & References

The microorganism collection and concentration by magnetic beads.

Figures 1.  Environmental microorganisms’ collection.  Add capture beads to 10ml of water sample spiked with 1x10^6 E. coli, mix and rotate at room temperature. Take an equal amount (200ul) of the mixture every 5 minutes and place it on a magnetic device for 2 minutes. Take 50ul of the supernatant and inoculate on the surface of the LB agar plate. Incubate plates at 37℃ for 18 hours and count the number of colonies. Plates (1-5) show that more bacteria are captured as the capture time increases. More than 99% of the bacteria are captured by the beads within 20 minutes. Plate 9 shows that the bacteria captured by the beads can be completely re-cultured. 

Genomic DNA Isolation using Auto-Mag® Water DNA/RNA Kit.

Figure 2. Genomic DNA from different volume of lake water sources was isolated with the Auto-Mag^® Water DNA/RNA Kit. DNA was extracted in triplicate from water samples as follows: sample 1-3 from 200 ml simples, sample 4-6 from 100 ml simples, sample 7-9 from 50 ml simples, and sample 10-12 from 15 ml simples. Genomic DNA was analyzed on a 1% agarose gel to demonstrate yield and quality of the DNA.

Excellent Quality

        Figure 3. Detection of isolated DNA by Real-Time PCR (SYBR                  Green). The E. coli carrying an ampicillin resistance plasmid was            added to the water sample. E. coli was captured, and DNA was              isolated using the Auto-Mag® Water DNA/RNA Kit. The purified             DNA was a serial 10-fold dilution for PCR amplification. The                     results showed that the purified DNA has high purity, and no                   obvious inhibitors were present.