Auto-Mag® HMW DNA Isolation Kit

Auto-Mag® HMW DNA Isolation Kit comes with modified beads and is designed for extraction of High-Molecular-Weight DNA in the 50 – 300+ kb size range from blood, saliva, or tissue cultured cells samples. The HMW DNA purified with this system is particularly suitable for analysis on long-range genomics platforms including PacBio RSII/Sequel/Sequel II and Oxford Nanopore.

The exact size of extracted DNA will vary depending on sample type, the quality of the starting material, and processing parameters. High quality samples are the key to obtaining high quality DNA. Either fresh or frozen samples can be used equivalently. However, care should be taken to minimize freeze-thaws and the time samples spend at ambient or at 4 °C.

Additionally, To maximize genomic DNA size, the protocols for this kit incorporate no using pipette mixing or vortex mixing during the extraction procedures except the elution step.

Features:

• Reproducibly purify high molecular weight genomic DNA (50-300+kb) from a wide range of samples matrices.
• Simplified, user friendly protocols that remove RNA and inhibitors.
• Comes with enzymes mixture for isolation high quality HMW DNA from Bacteria
• HMW DNA is suitable for all third-generation sequencing platforms including Nanopore and PacBio SMRT sequencing.
• Automation-friendly and can be easily scaled up.

Product Data & Figures

Product Documentation

Auto-Mag® HMW DNA Isolation Kit Protocols V.2.2

Auto-Mag® HMW DNA Isolation Kit MSDS

Certification of Analysis (COA)

Lot No. D03645078                   Lot No. D03645228

Frequently asked questions (FAQs)