Auto-Mag® Cell-free DNA (cfDNA) Isolation Kit

Auto-Mag® Cell-free DNA (cfDNA) Isolation Kit utilizes magnetic based technology and provides fast, reliable, and simple procedures for isolating the highest quality and quantity of cell-free circulating DNA (cfc-DNA) from various amounts of plasma/serum ranging from 0.4 mL up to 10 mL with minimal genomic DNA (gDNA) contamination. The kit is designed to isolate all sizes of cfc-DNA and ct-DNA from either fresh, preserved, or frozen plasma/serum samples. Moreover, this kit allows the user to elute the purified cfc-DNA into a flexible elution volume ranging from 25μl to 50μl. The purified p high-quality cfc-DNA is eluted in an Elution Buffer that is suitable for a broad range of applications, including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and DNA library preparation for next generation sequencing etc.

Highlights & Applications

Highlights:

• Efficient Isolation of circulating cell-free DNA from up to 10 ml or more of plasma
• No carrier RNA
• Low sample volume processing
• Efficient recovery of small sized fragmented DNA
• No organic extraction or ethanol precipitation
• Adaptable to various automated workstations

Product Documents

Auto-Mag® Cell-free DNA (cfDNA) Isolation Kit – Protocol

Auto-Mag® Cell-free DNA (cfDNA) Isolation Kit – MSDS

Additional Information

Certification of Analysis (COA)

Lot No. D01044706