Auto-Mag® DNA Normalization Kit

The Auto-Mag® DNA Normalization Kit is a paramagnetic bead-based kit. DNA normalization is based on the binding of DNA to proprietary beads of limited binding capacity; excess DNA is washed away, and a normalized amount of DNA is recovered. With this kit, the required uniform DNA concentration can be obtained from DNA samples of various concentrations without considering the concentration of the initial input DNA during the DNA normalization process, and without using fluorescence measurement or other methods; thus, saving time and operating costs. This protocol does not require a centrifugation step and can be performed in 96-well plates (manual and automated) and single tubes (manual only) using related protocols.

The kit also contains Auto-Mag® PCR-Pure reagent, which can be used in the recovery of unbound excess DNA during DNA normalization. Auto-Mag® PCR-Pure also can be used for PCR/DNA purification, or DNA fragment size selection for NGS library preparation. For more detail information, please reference Auto-Mag® PCR-Pure (Cat #: S002)

Highlights & Applications

Highlights:

• Rapid and reliable quantitation and normalization of DNA
• No centrifugation step, no filtration step
• Allowing the processing of pool DNA samples from various sources
• Equalizing input genomic DNA concentration for DNA library construction to help produce consistent and reliable NGS data without tedious initial input DNA quantitation.
• Reduce library construction time, reagents usage and overall costs

Product Documents

Auto-Mag® DNA Normalization Kit Protocols

Auto-Mag® DNA Normalization Kit MSDS

Additional Information

Certification of Analysis (COA)

Lot No. S00644615           Lot No. S00644693           Lot No. S00644761                 Lot No. S00645090