The Auto-Mag® DNA Normalization Kit is a paramagnetic bead-based kit. DNA normalization is based on the binding of DNA to proprietary beads of limited binding capacity; excess DNA is washed away, and a normalized amount of DNA is recovered. With this kit, the required uniform DNA concentration can be obtained from DNA samples of various concentrations without considering the concentration of the initial input DNA during the DNA normalization process, and without using fluorescence measurement or other methods; thus, saving time and operating costs. This protocol does not require a centrifugation step and can be performed in 96-well plates (manual and automated) and single tubes (manual only) using related protocols.
The kit also contains Auto-Mag® PCR-Pure reagent, which can be used in the recovery of unbound excess DNA during DNA normalization.
DNA quantitation and normalization of DNA concentration for: