Auto-Mag® DTR

Auto-Mag® DTR is consists of AMD Biotech’s own paramagnetic beads and compatible buffers and is designed to remove of unincorporated dye terminators effectively and reliably from Sanger sequencing reaction mixtures. The process consists of three simple steps including bind, wash and elute. While binding the sequencing product selectively to the paramagnetic beads, unincorporated dyes, nucleotides, salts and primers will be removed during ethanol washes. This principle allows for elution of the pure Sanger Sequencing product in the elution buffer of choice. Auto-Mag® DTR can be used for manual procedures as well as guidelines for adapting it to automatic liquid handling workstations (e.g. Dynamic Devices, Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent and Eppendorf)

Highlights & Applications

Highlights

• No protocol changes against major competitor.
• Longer Phred 20 read lengths averaging over 900 bases with a low retest rate
• Pass rates over 85% or higher
• Manual or adjustable to automated liquid handlers
• Reduce BigDye usage, due to increased average signal strength
• 96well – or 384-well formats
• Up to 95% significant cost savings compared to any similar products.

Application

Clean up of sequencing product for both ABI and MegaBACE platforms Supported Chemistries

• BigDye* versions 1.0, 1.1, 2.0, 3.0 and 3.1
• DYEnamic ET

*BigDye is a registered trademark of Applied Biosystems

Product Documents

Auto-Mag® DTR Protocol

Auto-Mag® DTR MSDS

Additional Information

Fig 1:  The Dye-blobs was removed by Auto-Mag® DTR.

Fig 2. pUC-19 plasmid was sequenced using the BigDye Terminator v3.1 Chemistry with  recommended protocols.  10 ul Sequencing reaction products was purified by using 10 ul Auto-Mag® DTR. DNA was analyzed on an Applied Biosystems’ 3730XL. The Sequencing reading  is over 1200bp. There is no dye blobs and Phred 20 read lengths is over 1000bp

Certification of Analysis ( COA )

Lot No. S00144375           Lot No. S00144568         Lot No. S00144970