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Auto-Mag® PCR-Pure
November 26, 2019
Auto-Mag® PCR Recovery and Clean-up Kit
November 26, 2019

Auto-Mag® DTR

$48.00$2,016.00

Magnetic beads based reagent for manual and automated Sanger sequencing reaction clean-up for both ABI and MegaBACE sequencing platforms.

Buy one get one 50% off now !                             Free Sample Kit upon request!

Catalog Item Description Price Quantity
AMD-S004-5 Auto Mag® DTR 5ml $48.00
AMD-S004-50 Auto Mag® DTR 50ml $346.00
AMD-S004-250 Auto Mag® DTR 250ml $1,275.00
AMD-S004-500 Auto Mag® DTR 500ml $2,016.00
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SKU: AMD-S004 Category:

Auto-Mag® DTR is a paramagnetic bead-based system designed for the removal of primer-dimers, salts, and unincorporated dye terminators from Sanger sequencing reaction mixtures.  The high sensitivity and binding ability of Auto-Mag® DTR allows for decreased concentrations of BigDye® chemistry to be used and longer continuous read lengths to be achieved. Auto-Mag® DTR can be processed in 96- and 384-well formats with three simple steps: bind, wash, and elute.  The protocol can be used for manual procedure as well as guideline for adapting it to automatic liquid handling workstations currently on the market (e.g. Beckman, Hamilton, Tecan, Beckman Courter, Caliper, Perkin Elmer, Agilent and Eppendorf)

Highlights

  • Designed for “bottle swap” with no protocol change against major competitor
  • Rapid and reliable clean-up of sequencing reactions
  • Longer contiguous sequence read lengths (>900 bases) with a low retest rate
  • No centrifugation step, no filtration step.
  • Increased average signal strength and reduce Big-Dye usage
  • Available in a wide range of formats – single sample, 8-well, 96-well and 384-well formats Cost Effective.
  • Save up to 80% to similar products.

Application

Clean up of sequencing product for both ABI and MegaBACE platforms

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Additional Information

Fig 1:  The Dye-blobs was removed by Auto-Mag® DTR.

Fig 2. PEGM plasmid was sequenced using the BigDye Terminator v3.1 Chemistry with  recommended protocols.  10 ul Sequencing reaction products was purified by using 10 ul Auto-Mag® DTR. DNA was analyzed on an Applied Biosystems’ 3730XL. The Sequencing reading  is over 1200bp. There is no dye blobs and Phred 20 read lengths is over 1000bp

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Protocols

Auto-Mag® DTR protocol

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MSDS

MSDS for Auto-Mag® DTR Clean-up System

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Certification of Analysis ( COA )

Date Lot Number Report
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