$48.00 – $2,016.00
Magnetic beads based reagent for manual and automated Sanger sequencing reaction clean-up for both ABI and MegaBACE sequencing platforms.
Buy one get one 50% off now ! Free Sample Kit upon request!
Auto-Mag® DTR is a paramagnetic bead-based system designed for the removal of primer-dimers, salts, and unincorporated dye terminators from Sanger sequencing reaction mixtures. The high sensitivity and binding ability of Auto-Mag® DTR allows for decreased concentrations of BigDye® chemistry to be used and longer continuous read lengths to be achieved. Auto-Mag® DTR can be processed in 96- and 384-well formats with three simple steps: bind, wash, and elute. The protocol can be used for manual procedure as well as guideline for adapting it to automatic liquid handling workstations currently on the market (e.g. Beckman, Hamilton, Tecan, Beckman Courter, Caliper, Perkin Elmer, Agilent and Eppendorf)
Clean up of sequencing product for both ABI and MegaBACE platforms
Fig 1: The Dye-blobs was removed by Auto-Mag® DTR.
Fig 2. PEGM plasmid was sequenced using the BigDye Terminator v3.1 Chemistry with recommended protocols. 10 ul Sequencing reaction products was purified by using 10 ul Auto-Mag® DTR. DNA was analyzed on an Applied Biosystems’ 3730XL. The Sequencing reading is over 1200bp. There is no dye blobs and Phred 20 read lengths is over 1000bp