$38.00 – $2,280.00
Magnetic beads-based chemistry for DNA clean up and fragment size selection of NGS library construction
Auto-Mag® PCR-Pure consists of AMD’s own paramagnetic beads and optimized chemicals that is specifically designed for selection of sheared DNA fragments and amplicon purification. Fragment size selection by Auto-Mag® PCR-Pure is always tight and consistent. The purification process consists of removal of salts, primers, primer-dimers, dNTPs, salts, enzymes, adapters, and adapter dimers DNA amplicons and fragments are selectively bound to the magnetic beads’ particles; and highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications. Auto-Mag® PCR-Pure is compatible with most next generation sequencing platforms.
DNA fragments purified with Auto-Mag® PCR-Pure are ready to be used in the following applications:
Fig. 1, Double –sided Dual size selection: The electropherogram overlay of the double-sided size selection on sheared gDNA at 0.7x/0.3 x ratio set using Auto-Mag® PCR-Pure and a comparable product from Company A following manufacturer’s recommended protocols. The eluted DNA was analyzed on Agilent’s Tape Station 2200.
Fig. 2, NGS library preparation. Auto-Mag® PCR-Pure performs double-sided size selection and purification on DNA from NGS library preparation.
Fig. 3. Percent recovery of 5 different sizes of DNA bands with the Auto-Mag® PCR-Pure according to manufacturer’s recommended protocols. The original input amounts of DNA (blue) were normalized in 100% and the amount of DNA recovered was determined by running on the 1.2% agarose gel and by optical density measurements with Nano Drop® 2000c.
Lot No. S00244880 Lot No. S00244416