Auto-Mag® PCR-Pure

Auto-Mag® PCR-Pure consists of AMD’s own paramagnetic beads and optimized chemicals that is designed for high-throughput clean up PCR and DNA fragments, or the size selection of DNA fragments in the library construction process for next generation sequencing (NGS) with high recovery rates. In the size selection process, Auto-Mag® PCR-Pure can selectively bind fragments based on the ratio of Auto-Mag® PCR-Pure reagent to sample. Altering the ratio gives the user the ability to selectively keep or discard undesired fragment sizes. Auto-Mag® PCR-Pure is suitable for both manual and fully automated processing and uses a simple 3 steps procedure: Bind-Wash-Elute. Purified DNA is ready for downstream applications including NGS, microarrays, automated fluorescent DNA sequencing, restriction enzyme digestion, and other applications.

When performing Auto-Mag® PCR-Pure protocol, a magnet or centrifuge is required to pellet the magnetic particles. If performing the protocol manually without access to a magnet, sample tubes or plate can be centrifuged for 30 seconds (single tubes: full speed; plates: 3,000 x g) to enable the magnetic particles to form a pellet. All processes are to be carried out at room temperature (15–25 °C).

Highlights / Application

Highlights

• Rapid and reliable post-PCR and post enzymatic reaction clean-up
• High recovery of amplicons or dsDNA fragments greater than 100bp
• Single or double size selection capability and uniform fragments size distribution for NGS
• Adaptable to high throughput liquid handling workstations
• Designed for “bottle swap” with no protocol change against major competitor
• Highly cost-effective, and available in bulk with volume discounts

Applications

DNA fragments purified with Auto-Mag^® PCR-Pure are ready to be used in the following applications:

• PCR
• NGS library preparation
• Amplicon size selection for NGS
• Microarrays
• Sanger sequencing
• Restriction enzyme digestions, adapter ligations
• Cloning

Product Documents

Auto-Mag® PCR-Pure Protocol

Auto-Mag® PCR-Pure MSDS

Additional Information

Fig. 1, Double –sided Dual size selection: The electropherogram overlay of the double-sided size selection on sheared gDNA at 0.7x/0.3 x ratio set using Auto-Mag® PCR-Pure and a comparable product from Company A following manufacturer’s recommended protocols. The eluted DNA was analyzed on Agilent’s Tape Station 2200.

Fig. 2, NGS library preparation. Auto-Mag® PCR-Pure performs double-sided size selection and purification on DNA from NGS library preparation.

Fig. 3. Percent recovery of 5 different sizes of DNA bands with the Auto-Mag® PCR-Pure according to manufacturer’s recommended protocols. The original input amounts of DNA (blue) were normalized in 100% and the amount of DNA recovered was determined by running on the 1.2% agarose gel and by optical density measurements with Nano Drop® 2000c.

Certification of Analysis (COA)

Lot No. S00244880

Lot No. S00244416