Auto-Mag® PCR-Pure

Auto-Mag® PCR-Pure consists of AMD’s own paramagnetic beads and optimized chemicals that is specifically designed for selection of sheared DNA fragments and amplicon purification. Fragment size selection by Auto-Mag® PCR-Pure is always tight and consistent. The purification process consists of removal of salts, primers, primer-dimers, dNTPs, salts, enzymes, adapters, and adapter dimers DNA amplicons and fragments are selectively bound to the magnetic beads’ particles; and highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications. Auto-Mag® PCR-Pure is compatible with most next generation sequencing platforms.

Highlights / Application

Highlights

• Rapid and reliable post-PCR and post enzymatic reaction clean-up
• High recovery of amplicons or dsDNA fragments greater than 100bp
• Single or double size selection capability and uniform fragments size distribution for NGS
• Adaptable to high throughput liquid handling workstations
• Designed for “bottle swap” with no protocol change against major competitor
• Highly cost-effective, and available in bulk with volume discounts

Applications

DNA fragments purified with Auto-Mag^® PCR-Pure are ready to be used in the following applications:

• PCR
• NGS library preparation
• Amplicon size selection for NGS
• Microarrays
• Sanger sequencing
• Restriction enzyme digestions, adapter ligations
• Cloning

Product Documents

Auto-Mag® PCR-Pure Protocols

Auto-Mag® PCR-Pure MSDS

Additional Information

Fig. 1, Double –sided Dual size selection: The electropherogram overlay of the double-sided size selection on sheared gDNA at 0.7x/0.3 x ratio set using Auto-Mag® PCR-Pure and a comparable product from Company A following manufacturer’s recommended protocols. The eluted DNA was analyzed on Agilent’s Tape Station 2200.

Fig. 2, NGS library preparation. Auto-Mag® PCR-Pure performs double-sided size selection and purification on DNA from NGS library preparation.

Fig. 3. Percent recovery of 5 different sizes of DNA bands with the Auto-Mag® PCR-Pure according to manufacturer’s recommended protocols. The original input amounts of DNA (blue) were normalized in 100% and the amount of DNA recovered was determined by running on the 1.2% agarose gel and by optical density measurements with Nano Drop® 2000c.

Certification of Analysis (COA)

Lot No. S00244880           Lot No. S00244416