Auto-Mag® PCR-Pure

Auto-Mag® PCR-Pure is designed for quick high-throughput purification of DNA fragments from PCR or NGS workflows with high recovery rates. Auto-Mag® PCR-Pure consists of AMD’s paramagnetic particles and optimized chemicals that selectively recovers amplicons greater than 100 bp with consistency and reliability.  It is also capable of selectively binding fragments depending on the reagent-to-sample ratio used, giving the user flexibility to perform left, right, or double-sided size selection. This product can be used for both manual and fully automated purification of DNA samples. Purified DNA is suitable for a variety of downstream applications such as NGS library preparation, microarrays, automated fluorescent sequencing as well as for post PCR clean-up of shorter amplicons or restriction enzyme digestion products.

Highlights

• Designed for “bottle swap” with no protocol change against major competitor.
• One reagent for PCR, DNA or cDNA purification and clean-up.
• Efficiently removes unincorporated dNTPs, primers, primer dimers and other contaminants.
• High recovery of amplicons or dsDNA fragments greater than 100bp.
• Double-sided size selection capability and uniform fragments size distribution.
• Manual and automation-friendly sample processing in 96- and 384-well formats.

Applications

DNA fragments purified with Auto-Mag^®   PCR-Pure are ready to be used in the following applications:

• PCR
• NGS library preparation
• Amplicon size selection for NGS
• Microarrays
• Sanger sequencing
• Restriction enzyme digestions, adapter ligations
• Cloning

Additional Information

Fig. 1, Double –sided Dual size selection: The electropherogram overlay of the double-sided size selection on sheared gDNA at 0.7x/0.3 x ratio set using Auto-Mag® PCR-Pure Plus and a comparable product from Company A following manufacturer’s recommended protocols. The eluted DNA was analyzed on Agilent’s Tape Station 2200.

Fig. 2, NGS library preparation. Auto-Mag® PCR-Pure performs double-sided size selection and purification on DNA from NGS library preparation.

Fig. 3. Percent recovery of 5 different sizes of DNA bands with the Auto-Mag® PCR-Pure according to manufacturer’s recommended protocols. The original input amounts of DNA (blue) were normalized in 100% and the amount of DNA recovered was determined by running on the 1.2% agarose gel and by optical density measurements with Nano Drop® 2000c.

Protocol

Auto-Mag® PCR-Pure Protocols

Basic Protocol for PCR or DNA clean-up

Protocol for PCR clean-up (96-well Plate Format)

Protocol for DNA clean-up and single size selection (96-well Plate Format)

Protocol for DNA double-sided size selection (96-well Plate Format)

Protocol for removing adapter dimers (96-well Plate Format)

MSDS

MSDS for Auto-Mag® PCR-Pure

Certification of Analysis (COA)