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Auto-Mag® X-Pure Select
November 26, 2019
Auto-Mag® DTR
November 26, 2019

Auto-Mag® PCR-Pure

$38.00$2,280.00

Magnetic beads-based chemistry for DNA clean up and fragment size selection of NGS library construction

Free Sample Kit upon request!                                                                                                     Bulk Quotation request! 

Catalog Item Description Price Quantity
AMD-S002-01 Auto Mag®PCR Pure (5 ml) $38.00
AMD-S002-02 Auto Mag®PCR Pure (50 ml) $198.00
AMD-S002-03 Auto Mag®PCR Pure (500 ml) $1,290.00
AMD-S002-04 Auto Mag®PCR Pure (1000 ml) $2,280.00
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SKU: AMD-S002 Categories: ,

Auto-Mag® PCR-Pure consists of AMD’s own paramagnetic beads and optimized chemicals that is specifically designed for selection of sheared DNA fragments and amplicon purification. Fragment size selection by Auto-Mag® PCR-Pure is always tight and consistent. The purification process consists of removal of salts, primers, primer-dimers, dNTPs, salts, enzymes, adapters, and adapter dimers DNA amplicons and fragments are selectively bound to the magnetic beads’ particles; and highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications. Auto-Mag® PCR-Pure is compatible with most next generation sequencing platforms.

 

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Highlights / Application

Highlights

  • Rapid and reliable post-PCR and post enzymatic reaction clean-up
  • High recovery of amplicons or dsDNA fragments greater than 100bp
  • Single or double size selection capability and uniform fragments size distribution for NGS
  • Adaptable to high throughput liquid handling workstations
  • Designed for “bottle swap” with no protocol change against major competitor
  • Highly cost-effective, and available in bulk with volume discounts

Applications

DNA fragments purified with Auto-Mag® PCR-Pure are ready to be used in the following applications:

  • PCR
  • NGS library preparation
  • Amplicon size selection for NGS
  • Microarrays
  • Sanger sequencing
  • Restriction enzyme digestions, adapter ligations
  • Cloning
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Product Documents

Auto-Mag® PCR-Pure Protocols

Auto-Mag® PCR-Pure MSDS

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Additional Information

Fig. 1, Double –sided Dual size selection: The electropherogram overlay of the double-sided size selection on sheared gDNA at 0.7x/0.3 x ratio set using Auto-Mag® PCR-Pure and a comparable product from Company A following manufacturer’s recommended protocols. The eluted DNA was analyzed on Agilent’s Tape Station 2200.

Fig. 2, NGS library preparation. Auto-Mag® PCR-Pure performs double-sided size selection and purification on DNA from NGS library preparation.

Fig. 3. Percent recovery of 5 different sizes of DNA bands with the Auto-Mag® PCR-Pure according to manufacturer’s recommended protocols. The original input amounts of DNA (blue) were normalized in 100% and the amount of DNA recovered was determined by running on the 1.2% agarose gel and by optical density measurements with Nano Drop® 2000c.

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Certification of Analysis (COA)

Lot No. S00244880           Lot No. S00244416

 

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