$45.00 – $1,568.00
Magnetic beads based reagent for selective binding of RNA or DNA fragments for use with total RNA clean-up and reliable size selection.
Auto-Mag® RNA-Pure consists of RNase free AMD’s paramagnetic particles and is optimized chemically for high-throughput RNA or cDNA cleanup and concentration after any enzymatic reaction: including RNA synthesis in vitro transcription, DNase I treatment, capping and labeling, and even after other purification methods such as phenol/chloroform extraction. The RNA after cleanup is beneficial for the following standard protocols such as RNA labeling, capping, Proteinase K treatment, DNase I treatment, RNA-Seq, and RT-qPCR. This protocol enables the recovery of micro RNA (miRNA), small RNA, and total RNA from the enzymatic reactions by concentrating the RNA from a diluted sample.
RNA can be cleaned up in various ways, including phenol/chloroform extraction, lithium chloride precipitation, agarose gel electrophoresis, or spin columns isolation. Magnetic bead-based cleanup offers a new more efficient tool to clean up the RNA and it can be used for both manual procedure as well as automatic liquid handling instruments to clean up and/or concentrate the sample of interest.
Auto-Mag® RNA-Pure also can be used for PCR/DNA clean-up and DNA size selection for NGS library preparation processing. Please reference protocols of AMD Auto-Mag® PCR-Pure.
Highlights
Applications
Basic Protocol for Total RNA Clean-up
Protocol for Separation and Recovery of Large RNAs and Small RNAs
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