$38.00 – $2,380.00
Magnetic beads-based chemistry for DNA clean up and fragment size selection of NGS library construction
Auto-Mag® X-Pure Select consists of AMD’s own paramagnetic beads and optimized chemicals that is designed for high-throughput clean up PCR, DNA fragments, RNA, or the size selection of DNA fragments in the library construction process for next generation sequencing (NGS) with high recovery rates. In the size selection process, Auto-Mag® X-Pure Select can selectively bind fragments based on the ratio of Auto-Mag® X-Pure Select reagent to sample. Altering the ratio gives the user the ability to selectively keep or discard undesired fragment sizes. Auto-Mag® X-Pure Select is suitable for both manual and fully automated processing and uses a simple 3 steps procedure: Bind-Wash-Elute. Purified DNA is ready for downstream applications including NGS, microarrays, automated fluorescent DNA sequencing, restriction enzyme digestion, and other applications.
When performing Auto-Mag® X-Pure Select protocol, a magnet or centrifuge is required to pellet the magnetic particles. If performing the protocol manually without access to a magnet, sample tubes or plate can be centrifuged for 30 seconds (single tubes: full speed; plates: 3,000 x g) to enable the magnetic particles to form a pellet. All processes are to be carried out at room temperature (15–25 °C).
DNA fragments purified with Auto-Mag® X-Pure Select are ready to be used in the following applications: