Auto-Mag® RNA-Pure

Auto-Mag® RNA-Pure consists of RNase free AMD’s paramagnetic particles and is optimized chemically for high-throughput RNA or cDNA cleanup and concentration after any enzymatic reaction: including RNA synthesis in vitro transcription, DNase I treatment, capping and labeling, and even after other purification methods such as phenol/chloroform extraction. The RNA after cleanup is beneficial for the following standard protocols such as RNA labeling, capping, Proteinase K treatment, DNase I treatment, RNA-Seq, and RT-qPCR. This protocol enables the recovery of micro RNA (miRNA), small RNA, and total RNA from the enzymatic reactions by concentrating the RNA from a diluted sample.

RNA can be cleaned up in various ways, including phenol/chloroform extraction, lithium chloride precipitation, agarose gel electrophoresis, or spin columns isolation. Magnetic bead-based cleanup offers a new more efficient tool to clean up the RNA and it can be used for both manual procedure as well as automatic liquid handling instruments to clean up and/or concentrate the sample of interest.

Auto-Mag® RNA-Pure also can be used for PCR/DNA clean-up and DNA size selection for NGS library preparation processing. Please reference protocols of AMD Auto-Mag® PCR-Pure.

Highlights

• Adaptable for High-throughput purification of total RNA, large and small RNAs, and cDNA.
• Efficient at concentrating total RNA and miRNA from diluted samples and elute in as little as 10 μl.
• RNA or cDNA Recover rate >90%, and is ready for all downstream applications including NGS, RNA-Seq library preparations, RT-qPCR, and hybridization, etc.
• Double-sided size selection capability
• No centrifugation or filtration needed, Manual and automation-friendly sample processing in 96-well formats

Applications

• PCR and RT-PCR reactions
• Transfection for RNAi Experiments
• Probes for microarray or microarray
• cDNA synthesis and labeling
• In vitro RNA synthesis

Additional Information

Protocol

Auto-Mag® RNA-Pure protocols

Basic Protocol for Total RNA Clean-up

Protocol for Separation and Recovery of Large RNAs and Small RNAs

MSDS

MSDS for Auto-Mag RNA-Pure

Certification of Analysis (COA)