Auto-Mag® X-Pure Select

Auto-Mag® X-Pure Select consists of AMD’s own paramagnetic beads and optimized chemicals that is designed for high-throughput clean up PCR, DNA fragments, RNA, or the size selection of DNA fragments in the library construction process for next generation sequencing (NGS) with high recovery rates.  In the size selection process, Auto-Mag® X-Pure Select can selectively bind fragments based on the ratio of Auto-Mag® X-Pure Select reagent to sample. Altering the ratio gives the user the ability to selectively keep or discard undesired fragment sizes. Auto-Mag® X-Pure Select is suitable for both manual and fully automated processing and uses a simple 3 steps procedure: Bind-Wash-Elute. Purified DNA is ready for downstream applications including NGS, microarrays, automated fluorescent DNA sequencing, restriction enzyme digestion, and other applications.

When performing Auto-Mag® X-Pure Select protocol, a magnet or centrifuge is required to pellet the magnetic particles. If performing the protocol manually without access to a magnet, sample tubes or plate can be centrifuged for 30 seconds (single tubes: full speed; plates: 3,000 x g) to enable the magnetic particles to form a pellet. All processes are to be carried out at room temperature (15–25 °C).

Highlight & Application

Highlights

• Single or double size selection capability and uniform fragments size distribution for NGS
• Rapid and reliable post-PCR and post enzymatic reaction clean-up
• High recovery of amplicons or dsDNA fragments greater than 100bp
• Adaptable to high throughput liquid handling workstations
• Designed for “bottle swap” with no protocol change against major competitor
• Highly cost-effective, and available in bulk with volume discounts

Applications

DNA fragments purified with Auto-Mag^®  X-Pure Select are ready to be used in the following applications:

• PCR
• NGS library preparation
• Amplicon size selection for NGS
• Microarrays
• Sanger sequencing
• Restriction enzyme digestions, adapter ligations
• Cloning

Product Documents

Auto-Mag® X-Pure Select Protocol

Auto-Mag® X-Pure Select MSDS

Additional Information

Certification of Analysis ( COA )

Lot No. S00344882

Lot No. S00344852