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Auto-Mag® PCR-Pure
November 26, 2019

Auto-Mag® RNA-Pure

$45.00$1,568.00

Magnetic beads based reagent for selective binding of RNA  or DNA  fragments for use with total RNA clean-up and reliable size selection.

Free Sample Kit upon request!

Catalog Item Description Price Quantity
AMD-S001-5 Auto Mag® RNA Pure (5ml) $45.00
AMD-S001-50 Auto Mag® RNA Pure (50ml) $258.00
AMD-S001-250 Auto Mag® RNA Pure (250ml) $966.00
AMD-S001-500 Auto Mag® RNA Pure (500ml) $1,568.00
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SKU: AMD-S001 Category:

Auto-Mag® RNA-Pure consists of RNase free AMD’s paramagnetic particles and is optimized chemically for high-throughput RNA or cDNA cleanup and concentration after any enzymatic reaction: including RNA synthesis in vitro transcription, DNase I treatment, capping and labeling, and even after other purification methods such as phenol/chloroform extraction. The RNA after cleanup is beneficial for the following standard protocols such as RNA labeling, capping, Proteinase K treatment, DNase I treatment, RNA-Seq, and RT-qPCR. This protocol enables the recovery of micro RNA (miRNA), small RNA, and total RNA from the enzymatic reactions by concentrating the RNA from a diluted sample.

RNA can be cleaned up in various ways, including phenol/chloroform extraction, lithium chloride precipitation, agarose gel electrophoresis, or spin columns isolation. Magnetic bead-based cleanup offers a new more efficient tool to clean up the RNA and it can be used for both manual procedure as well as automatic liquid handling instruments to clean up and/or concentrate the sample of interest.

Auto-Mag® RNA-Pure also can be used for PCR/DNA clean-up and DNA size selection for NGS library preparation processing. Please reference protocols of AMD Auto-Mag® PCR-Pure.

Highlights

  • Adaptable for High-throughput purification of total RNA, large and small RNAs, and cDNA.
  • Efficient at concentrating total RNA and miRNA from diluted samples and elute in as little as 10 μl.
  • RNA or cDNA Recover rate >90%, and is ready for all downstream applications including NGS, RNA-Seq library preparations, RT-qPCR, and hybridization, etc.
  • Double-sided size selection capability
  • No centrifugation or filtration needed, Manual and automation-friendly sample processing in 96-well formats

Applications

  • PCR and RT-PCR reactions
  • Transfection for RNAi Experiments
  • Probes for microarray or microarray
  • cDNA synthesis and labeling
  • In vitro RNA synthesis
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Additional Information

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Protocol

Auto-Mag® RNA-Pure protocols

Basic Protocol for Total RNA Clean-up

Protocol for Separation and Recovery of Large RNAs and Small RNAs

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MSDS

MSDS for Auto-Mag RNA-Pure

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Certification of Analysis (COA)

DATE LOT # Report
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