Auto-Mag® Mega-Select

Long-Read DNA sequencing platforms, with read lengths >10kb, require high molecular weight DNA; however, purification of such long DNA molecule is difficult and often returns fragments of various sizes, requiring some form of post-extraction cleanup procedure to recovery HMW DNA fragments. The Auto-Mag® Mega-Select provides a quick method to remove unwanted short DNA fragments and recover HMW DNA fragments from DNA samples.

Auto-Mag® Mega-Select consists of AMD Biotech’s own paramagnetic beads and compatible buffers. By simply adjusting the volume ratio of reagent to sample, DNA <5kb, <10kb, or <20kb in length can be quickly removed to trace levels from high molecular weight (HMW) DNA samples which leads to the recovery of large DNA fragments and significantly enhance mean read length of third-generation sequencing platforms, such as PacBio SMRT Technology or Oxford Nanopore Technology.

The Auto-Mag® Mega-Select Kit can be used to quickly cleanup unsheared HMW DNA samples and can also be used to selectively recover desired size HMW DNA fragments from fragmented HMW DNA samples or even partially degraded gDNA samples for library preparation of long-read sequencing (LRS). Since Auto-Mag® Mega-Select uses magnetic technology and requires no centrifugation or filtration, it is suitable for manual processing as well as adapting to automatic liquid handling workstations.

Features:

• Works with HMW DNA
• Effective purification and recovery of HMW DNA, dsDNA fragments 5kb or longer.
• Removal of short DNA fragments, impurities, and unwanted reaction components
• Fragment size selection of HMW DNA for specific applications of long-read sequencing.
• Expectant recover Yield: Up to 90%
• Compatible with manual and automated processing and cost effective.

Product Data & Figures

Product Documents

Auto-Mag® Mega-Select Protocol V 2.2.1

Auto-Mag® Mega-Select MSDS

Certification of Analysis (COA)

Lot No. S00545380                     Lot No. S00545231

Frequently asked questions (FAQs)