Auto-Mag® X-Pure Select consists of AMD’s own paramagnetic beads and optimized chemicals and is specifically designed for PCR amplicons cleaning up, DNA fragments, and RNA purification, or sheared DNA size selection in the library construction process for next generation sequencing (NGS).
Auto-Mag® X-Pure Select is an RNase-free reagent and can be used for RNA purification and clean-up. The PCR purification process consists of removal of salts, primers, primer-dimers, dNTPs, enzymes, adapters, and adapter dimers. The highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications. In DNA size selection process, based on the volume ratio of Auto-Mag® X-Pure Select reagent to sample, the desired size DNA Fragment are selected and recovered. Altering the ratio gives the user the ability to selectively keep or discard undesired fragment sizes. Auto-Mag® X-Pure Select is suitable for both manual and fully automated processing and is compatible with most next generation sequencing platforms.
Auto-Mag® X-Pure Select uses a magnetic bead-based chemistry for cleaning up DNA amplicons and binding of target DNA fragment of the desired size range. The kit is intended for robust and efficient amplicon clean-up and DNA fragment selection in next generation sequencing library preparation. Auto-Mag® X-Pure Select purified products can be used in the following applications:
Auto-Mag® X-Pure Select is also suitable for high-throughput purification of RNA or cDNA from in vitro applications such as transcription, antisense RNA (aRNA) amplification and RNA and cDNA probe synthesis. The purified RNA product can be used in the following applications: