Auto-Mag® X-Pure Select is based on paramagnetic bead technology, designed for sheared DNA fragment size selection of fragment library preparation for next generation sequencing (NGS), and purification of >100bp PCR amplicons.
In the DNA size selection, size selection is required to produce a uniform distribution of fragments around an average size. The protocols of DNA size selection describe procedures of DNA size selection by adjusting volume ratio of Auto-Mag® X-Pure Select reagent to sheared DNA sample and the desired size DNA fragment are selected and recovered by a simple binding, washing and elution steps.
For PCR products purification, the purification protocols provide the procedures for PCR purification in single tube or 96/384 well format. Auto-Mag® X-Pure Select reagent utilizes an optimized buffer to selectively bind DNA fragments >100 bp to the beads. Excess primers, nucleotides, salts, and enzymes can be removed using a simple washing procedure and PCR products are eluted with low salt elution buffer or water.
Auto-Mag® X-Pure Select is suitable for both manual and fully automated processing and the highly purified DNA can be used directly for downstream applications.