Auto-Mag® X-Pure Select

Auto-Mag® X-Pure Select is based on paramagnetic bead technology, designed for sheared DNA fragment size selection of fragment library preparation for next generation sequencing (NGS), and purification of >100bp PCR amplicons.

In the DNA size selection, size selection is required to produce a uniform distribution of fragments around an average size. The protocols of DNA size selection describe procedures of DNA size selection by adjusting volume ratio of Auto-Mag® X-Pure Select reagent to sheared DNA sample and the desired size DNA fragment are selected and recovered by a simple binding, washing and elution steps.

For PCR products purification, the purification protocols provide the procedures for PCR purification in single tube or 96/384 well format. Auto-Mag® X-Pure Select reagent utilizes an optimized buffer to selectively bind DNA fragments >100 bp to the beads. Excess primers, nucleotides, salts, and enzymes can be removed using a simple washing procedure and PCR products are eluted with low salt elution buffer or water.

Auto-Mag® X-Pure Select is suitable for both manual and fully automated processing and the highly purified DNA can be used directly for downstream applications.

Features:

• Works with fragmented DNA
• Single or double size selection capability and uniform fragments size distribution for NGS
• High recovery of amplicons or dsDNA fragments greater than 100bp
• 96- or 384-well formats
• Adaptable to high throughput liquid handling workstations
• Designed for “bottle swap” with no protocol change against major competitor.
• Significant cost savings

Product Data & Figures

Product Documents

Auto-Mag® X-Pure Select Protocols V.2.1

Auto-Mag® X-Pure Select MSDS

Certification of Analysis ( COA )

Lot No. S00345275                     Lot No. S00345170               Lot No. S00345046               Lot No. S00344882               Lot No. S00344852 

Frequently asked questions (FAQs)