Auto-Mag® X-Pure Select

Auto-Mag® X-Pure Select consists of AMD’s own paramagnetic beads and optimized chemicals and is specifically designed for PCR amplicons cleaning up, DNA fragments, and RNA purification, or sheared DNA size selection in the library construction process for next generation sequencing (NGS).

Auto-Mag® X-Pure Select is an RNase-free reagent and can be used for RNA purification and clean-up. The PCR purification process consists of removal of salts, primers, primer-dimers, dNTPs, enzymes, adapters, and adapter dimers. The highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications. In DNA size selection process, based on the volume ratio of Auto-Mag® X-Pure Select reagent to sample, the desired size DNA Fragment are selected and recovered. Altering the ratio gives the user the ability to selectively keep or discard undesired fragment sizes. Auto-Mag® X-Pure Select is suitable for both manual and fully automated processing and is compatible with most next generation sequencing platforms.

Highlight & Application

• Single or double size selection capability and uniform fragments size distribution for NGS
• Rapid and reliable post-PCR and post enzymatic reaction clean-up
• High recovery of amplicons or dsDNA fragments greater than 100bp
• Adaptable to high throughput liquid handling workstations
• Designed for “bottle swap” with no protocol change against major competitor
• Highly cost-effective, and available in bulk with volume discounts

Applications

Auto-Mag® X-Pure Select uses a magnetic bead-based chemistry for cleaning up DNA amplicons and binding of target DNA fragment of the desired size range. The kit is intended for robust and efficient amplicon clean-up and DNA fragment selection in next generation sequencing library preparation. Auto-Mag® X-Pure Select purified products can be used in the following applications:

• PCR
• Sequencing
• Genotyping
• Fragment Analysis
• Primer Walking, etc.

Auto-Mag® X-Pure Select is also suitable for high-throughput purification of RNA or cDNA from in vitro applications such as transcription, antisense RNA (aRNA) amplification and RNA and cDNA probe synthesis. The purified RNA product can be used in the following applications:

• PCR and RT-PCR,
• Probes for microarray,
• RNase protection assays,
• Transfection for RNAi experiments,
• cDNA synthesis and labeling. etc.

Product Documents

Auto-Mag® X-Pure Select Protocols

Auto-Mag® X-Pure Select MSDS

Additional Information

Certification of Analysis ( COA )

Lot No. S00345046               Lot No. S00344882               Lot No. S00344852